This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.Macrophages and microglia are the major cell types infected in the central nervous system of humans infected with HIV and macaques infected with SIV. Microglia are the resident macrophages of the brain and have been shown to be quite sensitive to even minor disturbances of central nervous system (CNS) homeostasis, and are readily activated. Activation of microglia induces changes in cellular morphology and in the expression of cell surface receptors. CD163 is a member of the scavenger receptor family with cysteine-rich domains (SCRC) identified as a receptor of haptoglobin-hemoglobin (Hp-Hb) complex and exclusively expressed by cells of monocyte-macrophage lineage. We examined the expression of CD163 in vitro and in vivo by multiple techniques and at varying times after SIV infection in animals with or without SIVE. Our data show that CD163 is expressed by cells of monocyte/macrophage lineage including perivascular macrophages but not parenchymal microglia in normal and acutely SIV-infected animals or animals with terminal AIDS without encephalitis. CD163 expression was detected in activated microglia (HLA-DR+) surrounding SIVE lesions in chronically infected macaques with severe encephalitis in the presence of haptoglobin-haemoglobin complex (Hp-Hb) in the tissue suggesting breakdown of the blood-brain-barrier. CD163 expression was also induced in microglia in vitro by stimulation with Hp-Hb complex indicating that the interaction of the Hp-Hb complex is required to trigger the upregulation of CD163. To confirm that activation of microglia was associated with the presence and upregulation of CD163 RNA we treated microglia isolated from rhesus macaque brain with Hp-Hb for 0, 6, 12, 18 and 48 h and performed quantitative real-time PCR. We observed a 2.5 fold increase of CD163 RNA in the microglia treated with haptoglobin-hemoglobin within 18hrs of exposure. We conclude that CD163 is a selective marker of perivascular macrophages in normal macaques and during the early phases of SIV infection. However, latter in infection CD163 also labels microglia that have been activated probably as a result of vascular compromise.

National Institute of Health (NIH)
National Center for Research Resources (NCRR)
Primate Research Center Grants (P51)
Project #
Application #
Study Section
Special Emphasis Panel (ZRR1-CM-8 (01))
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Tulane University
Other Domestic Higher Education
New Orleans
United States
Zip Code
Dutta, Noton K; McLachlan, James; Mehra, Smriti et al. (2014) Humoral and lung immune responses to Mycobacterium tuberculosis infection in a primate model of protection (.) Trials Vaccinol 3:47-51
Baliban, Scott M; Michael, Amanda; Shammassian, Berje et al. (2014) An optimized, synthetic DNA vaccine encoding the toxin A and toxin B receptor binding domains of Clostridium difficile induces protective antibody responses in vivo. Infect Immun 82:4080-91
Pan, Diganta; Kenway-Lynch, Carys S; Lala, Wendy et al. (2014) Lack of interleukin-10-mediated anti-inflammatory signals and upregulated interferon gamma production are linked to increased intestinal epithelial cell apoptosis in pathogenic simian immunodeficiency virus infection. J Virol 88:13015-28
Baker, Kate C; Bloomsmith, Mollie A; Oettinger, Brooke et al. (2014) Comparing options for pair housing rhesus macaques using behavioral welfare measures. Am J Primatol 76:30-42
Darrah, Patricia A; Bolton, Diane L; Lackner, Andrew A et al. (2014) Aerosol vaccination with AERAS-402 elicits robust cellular immune responses in the lungs of rhesus macaques but fails to protect against high-dose Mycobacterium tuberculosis challenge. J Immunol 193:1799-811
Parthasarathy, Geetha; Philipp, Mario T (2014) The MEK/ERK pathway is the primary conduit for Borrelia burgdorferi-induced inflammation and P53-mediated apoptosis in oligodendrocytes. Apoptosis 19:76-89
Ge, Dongxia; Zhang, Qing-Song; Zabaleta, Jovanny et al. (2014) Doublecortin may play a role in defining chondrocyte phenotype. Int J Mol Sci 15:6941-60
Walker, Joshua A; Sulciner, Megan L; Nowicki, Katherine D et al. (2014) Elevated numbers of CD163+ macrophages in hearts of simian immunodeficiency virus-infected monkeys correlate with cardiac pathology and fibrosis. AIDS Res Hum Retroviruses 30:685-94
Liu, David X; Gill, Amy; Holman, Patricia J et al. (2014) Persistent babesiosis in a Rhesus macaque (Macaca mulatta) infected with a simian-human immunodeficiency virus. J Med Primatol 43:206-8
Mohan, Mahesh; Chandra, Lawrance C; Torben, Workineh et al. (2014) miR-190b is markedly upregulated in the intestine in response to simian immunodeficiency virus replication and partly regulates myotubularin-related protein-6 expression. J Immunol 193:1301-13

Showing the most recent 10 out of 188 publications