To develop methodologies for the isolation of natural killer (NK) cells from the monkey uterus. RESULTS The mechanisms which promote the development of maternal-fetal immune tolerance in primates is not well-understood, but it has been hypothesized that one important component is the inhibition of uterine NK cells by trophblast MHC class I molecules. Previous studies demonstrated that the rhesus monkey placenta expresses a unique MHC class I molecule which has been designated Mamu-AG. In order to investigate the biological role of this molecule in primate pregnancy, we sought to establish methods for the isolation of rhesus monkey endometrium NK cells. Trypsin-DNase digestion of minced endometrial/decidual tissue proved to very efficiently disperse decidual cells, and gradient centrifugation yielded a population which was enriched (78%) in the NK marker CD56, and had low (10%) T/B cell contamination. Following in vitro enrichment, density gradient centrifugation yielded highly pure (>95%) CD56+ cells with less then 1% CD3+ lymphocytes. Uterine NK cells were effective at killing standard NK cell targets, including K562 and Raji cells. These results will be useful in developing approaches to examine rhesus trophoblast-uterine interactions in the establishment of maternal-fetal immune tolerance. FUTURE DIRECTIONS We will develop clones of uterine NK cells to define interactions between NK cell receptors and trophoblast MHC class I molecules. We will clone these receptors from NK cells and define their phylogenetic relationship with human and rodent NK cell receptors. KEY WORDS natural killer cell, maternal-fetal immune tolerance, endometrium, placenta, major histocompatibility complex

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Primate Research Center Grants (P51)
Project #
5P51RR000167-38
Application #
6277653
Study Section
Project Start
1998-05-01
Project End
1999-04-30
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
38
Fiscal Year
1998
Total Cost
Indirect Cost
Name
University of Wisconsin Madison
Department
Type
DUNS #
161202122
City
Madison
State
WI
Country
United States
Zip Code
53715
Kang, HyunJun; Mesquitta, Walatta-Tseyon; Jung, Ho Sun et al. (2018) GATA2 Is Dispensable for Specification of Hemogenic Endothelium but Promotes Endothelial-to-Hematopoietic Transition. Stem Cell Reports 11:197-211
Rhoads, Timothy W; Burhans, Maggie S; Chen, Vincent B et al. (2018) Caloric Restriction Engages Hepatic RNA Processing Mechanisms in Rhesus Monkeys. Cell Metab 27:677-688.e5
Ellis-Connell, Amy L; Balgeman, Alexis J; Zarbock, Katie R et al. (2018) ALT-803 Transiently Reduces Simian Immunodeficiency Virus Replication in the Absence of Antiretroviral Treatment. J Virol 92:
Park, Mi Ae; Jung, Ho Sun; Slukvin, Igor (2018) Genetic Engineering of Human Pluripotent Stem Cells Using PiggyBac Transposon System. Curr Protoc Stem Cell Biol 47:e63
Buechler, Connor R; Bailey, Adam L; Lauck, Michael et al. (2017) Genome Sequence of a Novel Kunsagivirus (Picornaviridae: Kunsagivirus) from a Wild Baboon (Papio cynocephalus). Genome Announc 5:
Wu, Hong; Whritenour, Jessica; Sanford, Jonathan C et al. (2017) Identification of MHC Haplotypes Associated with Drug-induced Hypersensitivity Reactions in Cynomolgus Monkeys. Toxicol Pathol 45:127-133
Shackman, A J; Fox, A S; Oler, J A et al. (2017) Heightened extended amygdala metabolism following threat characterizes the early phenotypic risk to develop anxiety-related psychopathology. Mol Psychiatry 22:724-732
Kalin, Ned H (2017) Mechanisms underlying the early risk to develop anxiety and depression: A translational approach. Eur Neuropsychopharmacol 27:543-553
Mattison, Julie A; Colman, Ricki J; Beasley, T Mark et al. (2017) Caloric restriction improves health and survival of rhesus monkeys. Nat Commun 8:14063
Feltovich, Helen (2017) Cervical Evaluation: From Ancient Medicine to Precision Medicine. Obstet Gynecol 130:51-63

Showing the most recent 10 out of 528 publications