This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.To maintain and further develop a specialized resource for studies relating to embryonic stem cell research.Allocation of Resource AccessTo date, the stem cell resource unit at the Wisconsin National Primate Research Center provides frozen rhesus and marmoset ES cells to interested investigators. No request has been denied. Additionally, the stem cell resource unit provides zebrafish bFGF for culturing primate ES cells. Since last submission 7 new investigators have received the recombinant protein bringing the total number of investigators receiving zbFGF on a regular basis to 12. Over 188mg of zbFGF has been distributed to date. This has saved investigators over $700,000. The DNA plasmid used to purify the protein itself is now available through Addgene (www.addgene.com) and was sent to 8 new investigators in 2007. DisseminationKnowledge is disseminated to the scientific community via publications in peer reviewed journals and scientific meeting attendance. The Wisconsin National Primate Research Center also holds quarterly research retreats to create increased communication between the various service and resource units.TrainingTraining in culture techniques of primate embryonic stem cells is available. Many new investigators have taken advantage of this resource in previous reporting periods however there have been no new investigators trained this year.ProgressCynomologous ES cell derivation: During 2007, the cynomologous cell line was frozen down and injected on two occasions into Rag1 mice. Approximately 5 X 10^6 cells were injected in each mouse. Unfortunately after three months no tumors were found and the mice were euthanized according to the animal protocol.Highlights:All four members of stem cell resources (IN CAPS) were authors on a ground breaking paper detailing reprogramming in human cells:Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells. JUNYING YU, Maxim A. Vodyanik, KIM SMUGA-OTTO, JESSICA ANTOSIEWICZ-BOURGET, Jennifer L. Frane, Shulan Tian, Jeff Nie, Gudrun A. Jonsdottir, Victor Ruotti, Ron Stewart, Igor I. Slukvin, and JAMES A. THOMSON Science 21 December 2007 318: 1917-1920; published online 19 November 2007.Challenges:Due to funding shortages we were unable to receive cynomologous embryos and the project came to a halt. We look forward to working with CPI to receive Mauritian cynomolougous embryos to create new cynomologous ES cell lines as described in our P51 proposal. Concerns:No concerns at this time.Publications note: Stem Cell Resource support is involved in numerous journal articles that depend in part or in full on WNPRC resources.
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