This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Phenotyping Core Laboratory (Core Unit A) will serve all projects on a highly interactive basis. Core Unit A has seven objectives. Objective 1 is to acquire, process, aliquot, and store blood samples as they come into the laboratory. On average, 426 serum and plasma samples/year will come in for processing. Objective 2 is to manage these and all other program-related samples currently in storage. Objective 3 is to quantify indicators of endothelial dysfunction in blood samples and culture medium. There will be an average of 213 blood samples/year and 326 samples/year of culture medium, in which as many as six endothelial functional markers will be assessed. The six biomarkers are E-selectin, endothelial nitric oxide synthase, and macrophage chemotactic protein 1, which will be assayed only in culture medium, and vascular cell adhesion molecule 1, intercellular adhesion molecule 1, and von Willibrand's factor, which will be assayed in both blood and culture medium samples. Objective 4 is to quantify, in an average of 139 samples per year, a panel of established risk factors for cardiovascular disease in blood samples from baboons undergoing an experiment that involves feeding an atherogenic diet for two years before a necropsy protocol for assessing extent of atherosclerotic lesions. The risk factors to be quantified include several analytes assayed using standard clinical chemistry methods (total and HDL cholesterol, triglyceride, apoAI, apoB, apoE, total antioxidant status, and C-reactive protein) and several specialized biochemical assays of established risk factors (lipoprotein size properties using gradient gel electrophoretic methods, enzyme activities of paraoxonase and lipoprotein-associated phospholipase A2, and concentrations of oxidized LDL). Objective 5 is to quantify extent of atherosclerotic lesions in artery samples taken at necropsy. Objective 6 is to establish new methodologies as required. Finally, Objective 7 is to collect and validate the data produced in this Core Unit and to make them available for analysis by Program investigators. The activities proposed for Core Unit A will provide a rich resource of samples and data that will be required to meet the Aims of the Projects in this Program.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Primate Research Center Grants (P51)
Project #
5P51RR013986-13
Application #
8357658
Study Section
Special Emphasis Panel (ZRR1-CM-8 (01))
Project Start
2011-05-01
Project End
2012-04-30
Budget Start
2011-05-01
Budget End
2012-04-30
Support Year
13
Fiscal Year
2011
Total Cost
$97,239
Indirect Cost
Name
Texas Biomedical Research Institute
Department
Type
DUNS #
007936834
City
San Antonio
State
TX
Country
United States
Zip Code
78245
Kumar, Shyamesh; Laurence, Hannah; Owston, Michael A et al. (2017) Natural pathology of the captive chimpanzee (Pan troglodytes): A 35-year review. J Med Primatol 46:271-290
Morosco, Danielle T; Cline, Curtis R; Owston, Michael A et al. (2017) Spontaneous mediastinal myeloid sarcoma in a common marmoset (Callithrix jacchus) and review of the veterinary literature. J Med Primatol 46:42-47
Muralimanoharan, Sribalasubashini; Li, Cun; Nakayasu, Ernesto S et al. (2017) Sexual dimorphism in the fetal cardiac response to maternal nutrient restriction. J Mol Cell Cardiol 108:181-193
Rodriguez-Sanchez, Iram P; Guindon, Josee; Ruiz, Marco et al. (2016) The endocannabinoid system in the baboon (Papio spp.) as a complex framework for developmental pharmacology. Neurotoxicol Teratol 58:23-30
Neidig, Lauren E; Owston, Michael A; Ball, Erin et al. (2016) Pauci-immune glomerulonephritis in a captive chimpanzee (Pan troglodytes), and a review of spontaneous cases in animals. J Med Primatol 45:336-341
Gharpure, Poorva; Kontogiorgos, Elias D; Opperman, Lynne A et al. (2016) Elastic Properties of Chimpanzee Craniofacial Cortical Bone. Anat Rec (Hoboken) 299:1718-1733
Schlabritz-Loutsevitch, Natalia; Gygax, Scott E; Dick Jr, Edward et al. (2016) Vaginal Dysbiosis from an Evolutionary Perspective. Sci Rep 6:26817
Ákos Szabó, C; Salinas, Felipe S; Li, Karl et al. (2016) Modeling the effective connectivity of the visual network in healthy and photosensitive, epileptic baboons. Brain Struct Funct 221:2023-33
Schlabritz-Loutsevitch, Natalia E; Comuzzie, Anthony G; Mahaney, Michael M et al. (2016) Serum Vitamin D Concentrations in Baboons (Papio spp.) during Pregnancy and Obesity. Comp Med 66:137-42
Bauer, Cassondra; Harrison, Tara (2016) Retrospective Analysis of the Incidence of Retained Placenta in 3 Large Colonies of NHP. Comp Med 66:143-9

Showing the most recent 10 out of 429 publications