The overall goal of the Genomics and Bioinformatics Core (GBC) of this Indiana Alcohol Research Center (lARC) is to support both human and animal studies that pursue the genes underlying alcoholpreference, alcoholism, and related diseases. The GBC has developed and deployed genotyping assays for the functional SNPs in human alcohol metabolizing enzymes {ADH1A, ADH1B, ADH1C, ALDH1A1, and ALDH2) and has determined the genotypes at these loci for many collaborative studies that have tested their association with alcoholism and related traits. We also design and offer SNP panels for genotyping the ADHs, GABA receptors, and other neurotransmitter receptors for association studies both within the lARC and for outside collaborators. The GBC has expanded its human genotyping services to include newly identified candidate genes at the request of collaborators. The GBC also offers DNA and RNA extraction services. To identify genes that are differentially expressed between high alcohol drinking and low alcohol drinking animal models, the Core will offer mRNA, microRNA, and protein quantification assays to confirm expression differences in candidate genes for alcoholism identified by microarrays, RNA-seq, or other technologies. Due to the enormous amount of data that needs to be analyzed in whole genome studies, the GBC will incorporate bioinformatics as one of our new services and provide data management, data analyses, data integration, and data sharing of large-scale data sets from next generation sequencing (RNA sequencing, whole genome DNA sequencing, and DNA-methylation analysis). In addition, the searchable data base, MyTrack, will benefit all the lARC investigators and registered users. The GBC will offer genetic monitoring for the alcohol preferring and nonpreferring rat lines and strains and provide alcohol concentration measurement using gas chromatography. The GBC supports human Components 2 and 3, Animal Research Components 5 and 6, pilot projects P58 and P59, numerous existing and future outside collaborations, the Animal Production Core for genetic monitoring, and all the lARC investigators for measuring alcohol concentrations. The GBC also interacts with the Administration Core. There is an increase in accuracy and cost savings as a result of having the genotyping and other services performed in a core laboratory rather than having multiple investigators set up independent facilities. The findings of the GBC contribute to the identification of genes that determine vulnerability to alcoholism, and will advance the understanding of alcohol-seeking behavior.

Public Health Relevance

The identification of genes, pathways, and behavior traits that determine vulnerability to alcoholism will advance our understanding of alcohol-preference difference and provide information for therapeutic treatments. The Genomics and Bioinformatics Core supplies vital support and key technologies that advance this goal.

Agency
National Institute of Health (NIH)
Institute
National Institute on Alcohol Abuse and Alcoholism (NIAAA)
Type
Comprehensive Center (P60)
Project #
2P60AA007611-26
Application #
8400544
Study Section
Special Emphasis Panel (ZAA1-GG (50))
Project Start
Project End
Budget Start
2012-12-25
Budget End
2013-11-30
Support Year
26
Fiscal Year
2013
Total Cost
$186,868
Indirect Cost
$67,081
Name
Indiana University-Purdue University at Indianapolis
Department
Type
DUNS #
603007902
City
Indianapolis
State
IN
Country
United States
Zip Code
46202
Wardell, Jeffrey D; Ramchandani, Vijay A; Hendershot, Christian S (2016) Drinking Motives Predict Subjective Effects of Alcohol and Alcohol Wanting and Liking During Laboratory Alcohol Administration: A Mediated Pathway Analysis. Alcohol Clin Exp Res 40:2190-2198
McClintick, Jeanette N; McBride, William J; Bell, Richard L et al. (2016) Gene Expression Changes in Glutamate and GABA-A Receptors, Neuropeptides, Ion Channels, and Cholesterol Synthesis in the Periaqueductal Gray Following Binge-Like Alcohol Drinking by Adolescent Alcohol-Preferring (P) Rats. Alcohol Clin Exp Res 40:955-68
Yoder, Karmen K; Albrecht, Daniel S; Dzemidzic, Mario et al. (2016) Differences in IV alcohol-induced dopamine release in the ventral striatum of social drinkers and nontreatment-seeking alcoholics. Drug Alcohol Depend 160:163-9
Bell, R L; Hauser, S; Rodd, Z A et al. (2016) A Genetic Animal Model of Alcoholism for Screening Medications to Treat Addiction. Int Rev Neurobiol 126:179-261
Sari, Youssef; Toalston, Jamie E; Rao, P S S et al. (2016) Effects of ceftriaxone on ethanol, nicotine or sucrose intake by alcohol-preferring (P) rats and its association with GLT-1 expression. Neuroscience 326:117-25
Ding, Zheng-Ming; Ingraham, Cynthia M; Rodd, Zachary A et al. (2016) Alcohol drinking increases the dopamine-stimulating effects of ethanol and reduces D2 auto-receptor and group II metabotropic glutamate receptor function within the posterior ventral tegmental area of alcohol preferring (P) rats. Neuropharmacology 109:41-8
O'Tousa, David S; Grahame, Nicholas J (2016) Long-Term Alcohol Drinking Reduces the Efficacy of Forced Abstinence and Conditioned Taste Aversion in Crossed High-Alcohol-Preferring Mice. Alcohol Clin Exp Res 40:1577-85
King, Andrea C; Hasin, Deborah; O'Connor, Sean J et al. (2016) A Prospective 5-Year Re-examination of Alcohol Response in Heavy Drinkers Progressing in Alcohol Use Disorder. Biol Psychiatry 79:489-98
Beckwith, Steven Wesley; Czachowski, Cristine Lynn (2016) Alcohol-Preferring P Rats Exhibit Elevated Motor Impulsivity Concomitant with Operant Responding and Self-Administration of Alcohol. Alcohol Clin Exp Res 40:1100-10
Qiu, Bin; Bell, Richard L; Cao, Yong et al. (2016) Npy deletion in an alcohol non-preferring rat model elicits differential effects on alcohol consumption and body weight. J Genet Genomics 43:421-30

Showing the most recent 10 out of 267 publications