The mission of the Animal Physiology Core is to assist investigators in the in vivo assessment of glucose and fatty acid metabolism, insulin sensitivity and energy homeostasis in mice and rats. It performs studies of body adiposity distribution and facilitates NMR spectroscopy, fMRI and microPET analysis and imaging of experimental models undergoing metabolic studies. The Core also provides specialized rodent surgeries for investigator laboratories and several cardiac functional assessments related to diabetic complications. Through collaborative efforts with the other Cores of the DRTC, the effects of defined pharmacological, dietary, environmental and genetic alterations are thoroughly characterized for their effects on glucose homeostasis, insulin action, and metabolism. To accomplish these goals, the Animal Physiology Core will: 1) advise investigators in designing metabolic studies relevant to the control of glucose homeostasis and insulin action in rodents;2) make available to investigators specialized measurements of whole body and tissue specific glucose sensitivity and insulin action including, but not limited to insulin, pancreatic and hyperglycemic clamp studies;3) provide specialized surgical models for the study of insulin sensitivity, energy balance, and glucose and fatty acid metabolism;4) offer instruction to students, postdoctoral fellows, investigators and technical staff in performing surgical and physiological techniques necessary to evaluate the controls of glucose homeostasis and insulin action;5) provide analysis of whole body carbohydrate/fatty acid oxidation, energy expenditure, feeding behavior, and locomotor activity using specialized metabolic (indirect calorimetry) and behavioral rodent cages;6) provide assessment of the effects of spontaneous (running wheel) or scheduled (treadmill) exercise on glucose homeostasis and metabolism;7) make available to investigators specialized measurements of rodent adipose tissue distribution using microCT and measurements of glycogen in liver and muscle, intrahepatic lipids and intramyocellular lipids using NMR;8) make available to investigators specialized measurements of brain energy and glucose utilization by functional MRI (fMRI) and microPET scanning;and 9) perform quantitative analyses of cardiac ischemia reperfusion injury and autonomic neuropathy. All these sen/ices are available to investigators new to diabetes research, as well as to investigators working on diabetes-related projects that can be enriched and extended by the use of the expertise and facilities of this core.

Public Health Relevance

The Animal Physiology Core provides cost effective, high quality resources that facilitate the scientific progress made by a large group of scientists committed to improve our understanding of the integrative physiology of diabetes in order to improve the treatment of patients with diabetes.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Comprehensive Center (P60)
Project #
5P60DK020541-37
Application #
8637046
Study Section
Special Emphasis Panel (ZDK1-GRB-2)
Project Start
Project End
Budget Start
2014-04-01
Budget End
2015-03-31
Support Year
37
Fiscal Year
2014
Total Cost
$369,154
Indirect Cost
$146,772
Name
Albert Einstein College of Medicine
Department
Type
DUNS #
110521739
City
Bronx
State
NY
Country
United States
Zip Code
10461
Rudolph, Bryan; Bjorklund, Nicole; Ovchinsky, Nadia et al. (2018) Methods to improve the noninvasive diagnosis and assessment of disease severity in children with suspected nonalcoholic fatty liver disease (NAFLD): Study design. Contemp Clin Trials 75:51-58
Tang, Yan; Kwon, Hyokjoon; Neel, Brian A et al. (2018) The fructose-2,6-bisphosphatase TIGAR suppresses NF-?B signaling by directly inhibiting the linear ubiquitin assembly complex LUBAC. J Biol Chem 293:7578-7591
Chemaly, Elie R; Troncone, Luca; Lebeche, Djamel (2018) SERCA control of cell death and survival. Cell Calcium 69:46-61
Iqbal, Niloy Jafar; Lu, Zhonglei; Liu, Shun Mei et al. (2018) Cyclin-dependent kinase 4 is a preclinical target for diet-induced obesity. JCI Insight 3:
Schloss, Jennifer; Ali, Riyasat; Racine, Jeremy J et al. (2018) HLA-B*39:06 Efficiently Mediates Type 1 Diabetes in a Mouse Model Incorporating Reduced Thymic Insulin Expression. J Immunol 200:3353-3363
Racine, Jeremy J; Stewart, Isabel; Ratiu, Jeremy et al. (2018) Improved Murine MHC-Deficient HLA Transgenic NOD Mouse Models for Type 1 Diabetes Therapy Development. Diabetes 67:923-935
Shu, Jun; Santulli, Gaetano (2018) Update on peripheral artery disease: Epidemiology and evidence-based facts. Atherosclerosis 275:379-381
Zhao, Xiaoping; Zhao, Li; Yang, Hao et al. (2018) Pyruvate kinase M2 interacts with nuclear sterol regulatory element-binding protein 1a and thereby activates lipogenesis and cell proliferation in hepatocellular carcinoma. J Biol Chem 293:6623-6634
Qiu, Yunping; Moir, Robyn D; Willis, Ian M et al. (2018) Enhanced Isotopic Ratio Outlier Analysis (IROA) Peak Detection and Identification with Ultra-High Resolution GC-Orbitrap/MS: Potential Application for Investigation of Model Organism Metabolomes. Metabolites 8:
Liu, Shunmei; Marcelin, Genevieve; Blouet, Clemence et al. (2018) A gut-brain axis regulating glucose metabolism mediated by bile acids and competitive fibroblast growth factor actions at the hypothalamus. Mol Metab 8:37-50

Showing the most recent 10 out of 559 publications