The mission of the Hormone Assay Core are to provide cost effective and efficient analyses of a variety of hormones, appropriate methodologies, cell lines and data storage venues to promote the Diabetes research efforts of the Einstein DRTC. Over the past two years, the Core has averaged 30,000 assays annually in support of 34 DRTC investigators. Productivity of the Core has been outstanding with 76 papers published that involved Core usage in the past 2.5 years. In addition, the appropriate physiological secretory function and insulin content of a variety of rodent mouse beta cell lines are characterized prior to supplying them to investigators at Einstein and to other investigator laboratories nationally and internationally. Thus, this Core has functioned as a local and national resource in support of diabetes-related research. The Core utilizies RIA, ELISA, and Luminex-based methodologies that have been established by the Core director and personnel. Data are pemianently archived and the results of assays are made available via a secure website accessible by investigators. These data are encrypted and access to the server is password protected. A charge back system is used to recover costs not covered by the DRTC budget. Education of investigators on the appropriate assay method is the function of the Director, and laboratory personnel instruct fellows and post-docs in the methodologies when appropriate. To accomplish these goals, the Hormone Assay Core will: 1) provide accurate, cost effective assays of a variety of hormones, adipokines and cytokines to DRTC investigators, using appropriate methodologies;2) assess insulin secretion from a series of mouse beta cell lines maintained by the Einstein DRTC for local use and distribution to the scientific community;3) provide an effective data storage and access system for providing results to investigators and monitoring Core use; 4) provide expert advice to DRTC investigators on Core use and education in assay performance and data interpretation;and 5) utilize the Core as a training platform for appropriate young diabetes investigators. Future goals are to enhance assay automation through shared purchase of a robot and bar-code compatible gamma counter, which will be used to enhance semi-automation of procedures. All these sen/ices are available to investigators new to diabetes research, as well as to investigators working on diabetes-related projects that can be enriched and extended by the use of the expertise and facilities of this core.

Public Health Relevance

The Hormone Assay Core provides cost effective, high quality assays that facilitate the scientific progress made by a large group of scientists committed to improve our understanding and treatment of patients with diabetes.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Comprehensive Center (P60)
Project #
5P60DK020541-37
Application #
8637048
Study Section
Special Emphasis Panel (ZDK1-GRB-2)
Project Start
Project End
Budget Start
2014-04-01
Budget End
2015-03-31
Support Year
37
Fiscal Year
2014
Total Cost
$265,185
Indirect Cost
$105,435
Name
Albert Einstein College of Medicine
Department
Type
DUNS #
110521739
City
Bronx
State
NY
Country
United States
Zip Code
10461
Carey, Michelle; Gospin, Rebekah; Goyal, Akankasha et al. (2017) Opioid Receptor Activation Impairs Hypoglycemic Counterregulation in Humans. Diabetes 66:2764-2773
Bilanges, Benoit; Alliouachene, Samira; Pearce, Wayne et al. (2017) Vps34 PI 3-kinase inactivation enhances insulin sensitivity through reprogramming of mitochondrial metabolism. Nat Commun 8:1804
Jatkar, Aditi; Kurland, Irwin J; Judex, Stefan (2017) Diets High in Fat or Fructose Differentially Modulate Bone Health and Lipid Metabolism. Calcif Tissue Int 100:20-28
Viant, Mark R; Kurland, Irwin J; Jones, Martin R et al. (2017) How close are we to complete annotation of metabolomes? Curr Opin Chem Biol 36:64-69
Zahalka, Ali H; Arnal-Estapé, Anna; Maryanovich, Maria et al. (2017) Adrenergic nerves activate an angio-metabolic switch in prostate cancer. Science 358:321-326
Jao, Jennifer; Powis, Kathleen M; Kirmse, Brian et al. (2017) Lower mitochondrial DNA and altered mitochondrial fuel metabolism in HIV-exposed uninfected infants in Cameroon. AIDS 31:2475-2481
Bowden, John A; Heckert, Alan; Ulmer, Candice Z et al. (2017) Harmonizing lipidomics: NIST interlaboratory comparison exercise for lipidomics using SRM 1950-Metabolites in Frozen Human Plasma. J Lipid Res 58:2275-2288
Seki, Yoshinori; Suzuki, Masako; Guo, Xingyi et al. (2017) In Utero Exposure to a High-Fat Diet Programs Hepatic Hypermethylation and Gene Dysregulation and Development of Metabolic Syndrome in Male Mice. Endocrinology 158:2860-2872
Nie, Wenna; Yan, Leyu; Lee, Yie H et al. (2016) Advanced mass spectrometry-based multi-omics technologies for exploring the pathogenesis of hepatocellular carcinoma. Mass Spectrom Rev 35:331-49
Han, Wenfei; Tellez, Luis A; Niu, Jingjing et al. (2016) Striatal Dopamine Links Gastrointestinal Rerouting to Altered Sweet Appetite. Cell Metab 23:103-12

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