The mission of the Analytical Imaging Core Is to assist DRTC investigators in routine and complex imaging technologies for their particular research needs. This is accomplished at several levels. First, the facility has traditionally emphasized teaching new users, on an individual basis, the imaging techniques they require for successful execution of their experiments. Appropriate staff effort is expended to address the needs of these laboratories by assisting them in experimental design, data collection, quantitative analysis and presentation. Secondly, the facility offers customized full service sample preparation for electron microscopy ranging from chemical fixation, embedding in resin and ultrathin sectioning, negative staining, immunogold labeling, critical point drying and metal shadowing. In addition, the facility offers a full range of low temperature techniques for electron microscopy including quick freezing in the millisecond range by plunge, metal mirror or high pressure freezing. Thirdly, the Analytical Imaging Core develops novel technologies and state-of-the-art methodologies to continually address the ongoing needs of the Einstein DRTC research laboratories. To accomplish these goals, the Analytical Imaging Core will: 1) advise investigators, trainees and staff on the best techniques and instrumentation to address their specific research problem;2) make available to investigators specialized light microscopy instrumentation for the analyses of cellular and molecular biological processes;3) provide dedicated electron microscopy expertise, service and training for the high resolution visualization of cellular, organelle, protein and nucleic acid structures;4) provide laboratory training of students, postdoctoral fellows, investigators and technical staff in performing techniques necessary to perform microscopy based analyses;5) assist in the appropriate data analysis for the evaluation of molecular targets and biological processes;and 6) continue the development of cutting-edge imaging methodologies and instrumentation. All these services are available to investigators new to diabetes research, as well as to investigators working on diabetes-related projects that can be enriched and extended by the use of the expertise and facilities of this core.

Public Health Relevance

The Analytical Imaging Core provides cost effective, high quality resources that facilitate the scientific progress made by a large group of scientists committed to improve our understanding of the molecular and cellular basis of diabetes in order to improve the treatment of patients with diabetes.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Comprehensive Center (P60)
Project #
5P60DK020541-37
Application #
8637049
Study Section
Special Emphasis Panel (ZDK1-GRB-2)
Project Start
Project End
Budget Start
2014-04-01
Budget End
2015-03-31
Support Year
37
Fiscal Year
2014
Total Cost
$8,399
Indirect Cost
$3,339
Name
Albert Einstein College of Medicine
Department
Type
DUNS #
110521739
City
Bronx
State
NY
Country
United States
Zip Code
10461
Carey, Michelle; Gospin, Rebekah; Goyal, Akankasha et al. (2017) Opioid Receptor Activation Impairs Hypoglycemic Counterregulation in Humans. Diabetes 66:2764-2773
Bilanges, Benoit; Alliouachene, Samira; Pearce, Wayne et al. (2017) Vps34 PI 3-kinase inactivation enhances insulin sensitivity through reprogramming of mitochondrial metabolism. Nat Commun 8:1804
Jatkar, Aditi; Kurland, Irwin J; Judex, Stefan (2017) Diets High in Fat or Fructose Differentially Modulate Bone Health and Lipid Metabolism. Calcif Tissue Int 100:20-28
Viant, Mark R; Kurland, Irwin J; Jones, Martin R et al. (2017) How close are we to complete annotation of metabolomes? Curr Opin Chem Biol 36:64-69
Zahalka, Ali H; Arnal-Estapé, Anna; Maryanovich, Maria et al. (2017) Adrenergic nerves activate an angio-metabolic switch in prostate cancer. Science 358:321-326
Jao, Jennifer; Powis, Kathleen M; Kirmse, Brian et al. (2017) Lower mitochondrial DNA and altered mitochondrial fuel metabolism in HIV-exposed uninfected infants in Cameroon. AIDS 31:2475-2481
Bowden, John A; Heckert, Alan; Ulmer, Candice Z et al. (2017) Harmonizing lipidomics: NIST interlaboratory comparison exercise for lipidomics using SRM 1950-Metabolites in Frozen Human Plasma. J Lipid Res 58:2275-2288
Seki, Yoshinori; Suzuki, Masako; Guo, Xingyi et al. (2017) In Utero Exposure to a High-Fat Diet Programs Hepatic Hypermethylation and Gene Dysregulation and Development of Metabolic Syndrome in Male Mice. Endocrinology 158:2860-2872
Nie, Wenna; Yan, Leyu; Lee, Yie H et al. (2016) Advanced mass spectrometry-based multi-omics technologies for exploring the pathogenesis of hepatocellular carcinoma. Mass Spectrom Rev 35:331-49
Han, Wenfei; Tellez, Luis A; Niu, Jingjing et al. (2016) Striatal Dopamine Links Gastrointestinal Rerouting to Altered Sweet Appetite. Cell Metab 23:103-12

Showing the most recent 10 out of 546 publications