The Cell and Virus Core is designed to support two major activities of the NGEC: cell separations andanalysis, and construction and production of self inactivating non-integrating lentiviral (NIL) vectors and viralstocks. AutoMACs and flow cytometry and sorting will be heavily utilized in Components 2-5 for LHE design,and in Components 6-8 for analysis of fluorescent reporter assays and immune function in in vivo genetargeting and repair experiments. NIL vectors will be used for delivery of LHE's and donor templates inselected experiments from Component 1, and in virtually all experiments proposed in Components 6-8.

National Institute of Health (NIH)
National Heart, Lung, and Blood Institute (NHLBI)
Linked Center Core Grant (PL1)
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Special Emphasis Panel (ZRR1-SRC (99))
Program Officer
Thomas, John
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Seattle Children's Hospital
United States
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