The Cell and Virus Core is designed to support two major activities of the NGEC: cell separations andanalysis, and construction and production of self inactivating non-integrating lentiviral (NIL) vectors and viralstocks. AutoMACs and flow cytometry and sorting will be heavily utilized in Components 2-5 for LHE design,and in Components 6-8 for analysis of fluorescent reporter assays and immune function in in vivo genetargeting and repair experiments. NIL vectors will be used for delivery of LHE's and donor templates inselected experiments from Component 1, and in virtually all experiments proposed in Components 6-8.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Linked Center Core Grant (PL1)
Project #
1PL1HL092557-01
Application #
7466790
Study Section
Special Emphasis Panel (ZRR1-SRC (99))
Program Officer
Thomas, John
Project Start
2007-09-30
Project End
2012-06-30
Budget Start
2007-09-30
Budget End
2008-06-30
Support Year
1
Fiscal Year
2007
Total Cost
$827,340
Indirect Cost
Name
Seattle Children's Hospital
Department
Type
DUNS #
048682157
City
Seattle
State
WA
Country
United States
Zip Code
98105
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