The Cell and Virus Core is designed to support two major activities of the NGEC: cell separations and analysis, and construction and production of self inactivating non-integrating lentiviral (NIL) vectors and viral stocks. AutoMACs and flow cytometry and sorting will be heavily utilized in Components 2-5 for LHE design, and in Components 6-8 for analysis of fluorescent reporter assays and immune function in in vivo gene targeting and repair experiments. NIL vectors will be used for delivery of LHE's and donor templates in selected experiments from Component 1, and in virtually all experiments proposed in Components 6-8.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Linked Center Core Grant (PL1)
Project #
5PL1HL092557-05
Application #
8121472
Study Section
Special Emphasis Panel (ZRR1-SRC (99))
Program Officer
Thomas, John
Project Start
2007-09-30
Project End
2013-06-30
Budget Start
2011-07-01
Budget End
2013-06-30
Support Year
5
Fiscal Year
2011
Total Cost
$845,477
Indirect Cost
Name
Seattle Children's Hospital
Department
Type
DUNS #
048682157
City
Seattle
State
WA
Country
United States
Zip Code
98105
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