Abstract

In vitro and in vivo techniques will be used to study the effects of ethanol (ETOH) on the hypothalamo-hypophysial-luteinizing hormone (LH) axis. The in vitro studies will be used to determine the effects of ETOH alone or in combination with either dopamine (DA), norepinephrine (NE) or prostaglandin E2 (PGE2) on the release of radioimmunoassayable luteinizing hormone-releasing hormone (LHRH) from isolated male rat median eminences (ME). For the in vivo studies, radioimmunoassay (RIA) and immunocytochemical (ICC) techniques will be used to determine the effects of ETOH administration of LHRH concentration of the hypothalamus and ME of normal, ovariectomized and pregnant rats. These correlative techniques will also be used to ascertain any effects that prenatal ETOH exposure may have on LHRH production and/or secretion patterns in fetal and early postnatal animals. Light and electron microscopic ICC techniques for LHRH will be used to visualize any changes which might occur in discrete regions of the hypothalamus and ME that may be affected by ETOH, whereas RIA will determine any quantitative changes in hypothalamic LHRH content. Also, RIA for LH will be used to evaluate any changes which might occur in the blood. In addition, electron microscopic ICC will be used to determine any subcellular changes in LHRH perikarya. This combined qualitative and quantitative approach will allow us to evaluate any endocrine changes due to ETOH, as well as add to our overall understanding of the hypothalamo-hypophysial control system as it relates to neuroendocrinology and reproduction.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute on Alcohol Abuse and Alcoholism (NIAAA)
Type
Research Project (R01)
Project #
5R01AA006014-03
Application #
3109245
Study Section
Reproductive Biology Study Section (REB)
Project Start
1983-05-01
Project End
1986-11-30
Budget Start
1985-05-01
Budget End
1986-11-30
Support Year
3
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of Texas Sw Medical Center Dallas
Department
Type
Overall Medical
DUNS #
City
Dallas
State
TX
Country
United States
Zip Code
75390

  Publications

Franci, C R; Anselmo-Franci, J A; Kozlowski, G P et al. (1993) Actions of endogenous vasopressin and oxytocin on anterior pituitary hormone secretion. Neuroendocrinology 57:693-9
Kozlowski, G P; Sterzl, I; Nilaver, G (1992) Localization patterns for immunoglobulins and albumins in the brain suggest diverse mechanisms for their transport across the blood-brain barrier (BBB). Prog Brain Res 91:149-54
Kozlowski, G P; Nilaver, G; Zlokovic, B V (1990) Immunoneurology: a serum protein afferent limb to the CNS. Adv Exp Med Biol 274:345-70
Iwamoto, G A; Mitchell, J H; Sadeq, M et al. (1989) Localization of tyrosine hydroxylase and phenylethanolamine N-methyltransferase immunoreactive cells in the medulla of the dog. Neurosci Lett 107:12-8
Dees, W L; Skelley, C W; Rettori, V et al. (1988) Influence of ethanol on growth hormone secretion in adult and prepubertal female rats. Neuroendocrinology 48:495-9
Rettori, V; Skelley, C W; McCann, S M et al. (1987) Detrimental effects of short-term ethanol exposure on reproductive function in the female rat. Biol Reprod 37:1089-96
Dees, W L; Ahmed, C E; Ojeda, S R (1986) Substance P- and vasoactive intestinal peptide-containing fibers reach the ovary by independent routes. Endocrinology 119:638-41
Dees, W L; Kozlowski, G P; Dey, R et al. (1985) Evidence for the existence of substance P in the prepubertal rat ovary. II. Immunocytochemical localization. Biol Reprod 33:471-6
Dees, W L; Rettori, V; Kozlowski, G P et al. (1985) Ethanol and the pulsatile release of luteinizing hormone, follicle stimulating hormone and prolactin in ovariectomized rats. Alcohol 2:641-6
Dees, W L; Skelley, C W; Kozlowski, G P (1985) Central effects of an antagonist and an antiserum to substance P on serum gonadotropin and prolactin secretion. Life Sci 37:1627-31

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