Agency
National Institute of Health (NIH)
Institute
National Institute on Alcohol Abuse and Alcoholism (NIAAA)
Type
Research Project (R01)
Project #
5R01AA006221-13
Application #
2043412
Study Section
Biochemistry, Physiology and Medicine Subcommittee (ALCB)
Project Start
1983-12-01
Project End
1996-11-30
Budget Start
1995-12-01
Budget End
1996-11-30
Support Year
13
Fiscal Year
1996
Total Cost
Indirect Cost
Name
University of Michigan Ann Arbor
Department
Biochemistry
Type
Schools of Medicine
DUNS #
791277940
City
Ann Arbor
State
MI
Country
United States
Zip Code
48109
Vatsis, Kostas P; Coon, Minor J (2005) Oxidative aldehyde deformylation catalyzed by NADPH-cytochrome P450 reductase and the flavoprotein domain of neuronal nitric oxide synthase. Biochem Biophys Res Commun 337:1107-11
Vatsis, Kostas P; Peng, Hwei-Ming; Coon, Minor J (2005) Abolition of oxygenase function, retention of NADPH oxidase activity, and emergence of peroxidase activity upon replacement of the axial cysteine-436 ligand by histidine in cytochrome P450 2B4. Arch Biochem Biophys 434:128-38
Vatsis, Kostas P; Peng, Hwei-Ming; Coon, Minor J (2002) Replacement of active-site cysteine-436 by serine converts cytochrome P450 2B4 into an NADPH oxidase with negligible monooxygenase activity. J Inorg Biochem 91:542-53
Peng, H M; Coon, M J (2000) Promoter function and the role of cytokines in the transcriptional regulation of rabbit CYP2E1 and CYP2E2. Arch Biochem Biophys 382:129-37
Vaz, A D; McGinnity, D F; Coon, M J (1998) Epoxidation of olefins by cytochrome P450: evidence from site-specific mutagenesis for hydroperoxo-iron as an electrophilic oxidant. Proc Natl Acad Sci U S A 95:3555-60
Coon, M J; Vaz, A D; McGinnity, D F et al. (1998) Multiple activated oxygen species in P450 catalysis: contributions To specificity in drug metabolism. Drug Metab Dispos 26:1190-3
Peng, H M; Coon, M J (1998) Regulation of rabbit cytochrome P450 2E1 expression in HepG2 cells by insulin and thyroid hormone. Mol Pharmacol 54:740-7
Jiang, Y; Kuo, C L; Pernecky, S J et al. (1998) The detection of cytochrome P450 2E1 and its catalytic activity in rat testis. Biochem Biophys Res Commun 246:578-83
Kuo, C L; Vaz, A D; Coon, M J (1997) Metabolic activation of trans-4-hydroxy-2-nonenal, a toxic product of membrane lipid peroxidation and inhibitor of P450 cytochromes. J Biol Chem 272:22611-6
Pernecky, S J; Coon, M J (1996) N-terminal modifications that alter P450 membrane targeting and function. Methods Enzymol 272:25-34

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