Reticulon/Nogo (RTN/Nogo) proteins are critical factors in neurological and neurodegenerative disorders. In our previous funding cycle, we investigated potential roles of neuronal RTN3 in the negative modulation of BACE1 proteolytic activity. We demonstrated that increased expression of RTN3 in mice reduced amyloid deposition in cortical brain regions. However, this increased expression of RTN3 also facilitated the formation of RTN3 immunoreactive dystrophic neurites (RIDNs) in the hippocampus, and the occurrence of RIDNs impaired hippocampal synaptic function. These results suggest that RTN3 can play two opposing roles in the formation of neuritic plaques, in which amyloid deposits are often surrounded by various dystrophic neurites (including RIDNs) and reactive glial cells. In this proposal, we aim to investigate how these two seemingly opposing roles can be dissociated. We hypothesize that somatodendritic RTN3 modulates BACE1 activity, and that aggregated axonal RTN3 facilitates the formation of dystrophic neurites. We will perform a set of experiments utilizing four animal models (Tg-RTN3 mice, RTN3 transgenic mice under Tet-Off inducible promoter, RTN1 and RTN3 KO mice) generated in our lab to test this hypothesis, specifically addressing the following specific aims: 1) To investigate the effect of RTN protein levels on the formation RIDNs;2) To explore the pathophysiological consequence of RIDNs;3) To determine the in vivo role of RTN proteins in the formation of neuritic plaques. Moreover, we have found that RIDNs naturally occur in the elderly mouse brain. To further understand their occurrence in elderly human brains, we will address an additional specific aim, to identify and characterize RIDNs in human brains. Postmortem brains from at least two different sources will be used to address this specific aim. We will determine whether RIDNs occur in elderly human brains and whether this occurrence is significantly more frequent in AD brains. The results from the above studies will provide further evidence that RIDNs represent an early stage of dystrophic neurites in elderly and AD brains and that reducing RIDNs is an important novel drug target with the aim of improving cognitive function in the elderly population and AD patients.
Extracellular neuritic (senile) plaques and intraneuronal neurofibrillary tangles are two known pathological hallmarks in brains of patients with Alzheimer's disease (AD). Neuritic plaques are mainly composed of amyloid deposits that are frequently associated with dystrophic neurites and reactive glial cells. Our research is focused on the regulated formation of amyloid deposition and dystrophic neurites. Specifically, we investigated the role of reticulon proteins (RTNs) in AD pathogenesis in the previous funding cycle, and demonstrated that RTNs function as negative modulators of BACE1, a critical enzyme that cleaves amyloid precursor protein (APP) at the b-secretase site, contributing to the release of AB, the major component in amyloid deposits. We further demonstrated that the primary RTN that modulates BACE1 is neuronal reticulon 3 (RTN3). However, we also showed that increased expression of RTN3 can facilitate the formation of RTN3 immunoreactive dystrophic neurites (RIDNs), which are present in an abundant form primarily surrounding amyloid deposits in AD brains. These contrasting results suggest that RTN3 has a dual role in the formation of neuritic plaques. In this proposal, we aim to investigate how these two opposing effects can be dissociated. Our central hypothesis is that excessive aggregation of RTN3 in axons leads to the formation of RIDNs, whereas monomeric RTN3 in somoatodendrites modulates BACE1 activity. To test this hypothesis, we will perform a series of complimentary in vitro and in vivo experiments with four major aims: 1) To investigate the effects of RTN protein levels and aggregation on the formation RIDNs;2) To explore the pathophysiological consequences of RIDNs;3) To determine the in vivo role of RTN proteins in the formation of neuritic plaques;4) To identify and characterize RIDNs in human brains. Therefore, these studies will help reveal the role of RTN3 in the formation of amyloid deposition and dystrophic neurites using four different animal models, and will also contribute to our understanding of the occurrence of RIDNs in human brains. The knowledge gained from this series of studies will determine the importance of RTNs (specifically RTN3) in the formation of dystrophic neurites and will be useful in the development of therapeutic agents and strategies to inhibit the formation of neuritic plaques that are associated with RTN3 aggregation.
|Shi, Qi; Ge, Yingying; Sharoar, Md Golam et al. (2014) Impact of RTN3 deficiency on expression of BACE1 and amyloid deposition. J Neurosci 34:13954-62|
|He, Wanxia; Hu, Jinxuan; Xia, Yuxing et al. (2014) ?-site amyloid precursor protein cleaving enzyme 1(BACE1) regulates Notch signaling by controlling the cleavage of Jagged 1 (Jag1) and Jagged 2 (Jag2) proteins. J Biol Chem 289:20630-7|
|Yan, Riqiang; Vassar, Robert (2014) Targeting the ? secretase BACE1 for Alzheimer's disease therapy. Lancet Neurol 13:319-29|
|Zhao, Bei; Pan, Bai-Shen; Shen, Su-Wen et al. (2013) Diabetes-induced central neuritic dystrophy and cognitive deficits are associated with the formation of oligomeric reticulon-3 via oxidative stress. J Biol Chem 288:15590-9|
|Shi, Qi; Prior, Marguerite; Zhou, Xiangdong et al. (2013) Preventing formation of reticulon 3 immunoreactive dystrophic neurites improves cognitive function in mice. J Neurosci 33:3059-66|
|Hu, Xiangyou; He, Wanxia; Luo, Xiaoyang et al. (2013) BACE1 regulates hippocampal astrogenesis via the Jagged1-Notch pathway. Cell Rep 4:40-9|
|Deng, Minzi; He, Wanxia; Tan, Ya et al. (2013) Increased expression of reticulon 3 in neurons leads to reduced axonal transport of * site amyloid precursor protein-cleaving enzyme 1. J Biol Chem 288:30236-45|
|Zhou, Xiangdong; Hu, Xiangyou; He, Wanxia et al. (2011) Interaction between amyloid precursor protein and Nogo receptors regulates amyloid deposition. FASEB J 25:3146-56|
|Luo, Xiaoyang; Prior, Marguerite; He, Wanxia et al. (2011) Cleavage of neuregulin-1 by BACE1 or ADAM10 protein produces differential effects on myelination. J Biol Chem 286:23967-74|
|Hu, Xiangyou; Zhou, Xiangdong; He, Wanxia et al. (2010) BACE1 deficiency causes altered neuronal activity and neurodegeneration. J Neurosci 30:8819-29|
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