Alzheimer?s disease (AD) is characterized by the deposition of amyloid Bprotein (AB), a small peptide derived from B- and y-secretase cleavages of the amyloid precursor protein (APP). We recently demonstrated that the last 37 amino acids (LRP-C37) of low-density lipoprotein receptor-related protein (LRP) without the NPXY motifs to be necessary and sufficient to increase ABproduction. Since LRP-C37 alone was a potent inducer of ABproduction, we used this domain as bait in a yeast 2-hybrid screen, resulting in the identification of Ranbinding protein M (RanBP9). Indeed transient transfections of APP and RanBP9-FL or FL-derived RanBP9- N60 robustly increased secretion of ABin varieties of cell lines, indicating that RanBP9 alters APP metabolism. Most importantly, immunoblot quantification of RanBP9 protein levels demonstrated that RanBP9-N60 and RanBP9-FL were elevated more than six and four-folds in the brains of AD patients and APP J20 transgenic mice respectively. We also found that like LRP and two of its key ligands, RanBP9 is genetically associated with late-onset AD. To gain a better insight on the in vivo role of RanBP9 in the pathogenesis of AD, we generated transgenic mice over expressing RanBP9 in the brain as a part of an ongoing NIH R03 grant. By crossing B6C3-Tg85Dbo mice (APdE9) carrying APPswe, PSEN1dE9 mutations with RanBP9 transgenic mice, RanBP9/APdE9 triple transgenic mice were generated, which produced more CHAPSO-soluble ABand c-terminal fragments (CTFs) compared to APdE9 mice as early as 3 months of age, suggesting that RanBP9 increases amyloidogenic processing of APP in vivo. This R01 proposal is an extension of the R03 project. As loss of synapses is a better correlate of the extent of cognitive deficits in Alzheimer?s patients and since RanBP9 is present in substantial amounts in neurites and is a strong inhibitor of neurite outgrowth, we next want to examine in this proposal, whether RanBP9-induced altered processing of APP also leads to dendritic and spine injury. We have successfully produced RanBP9 transgenic mice as well as heterozygous null mice for the first time. We propose to compare the pattern of dendritic arborization, spine density, presynaptic and postsynaptic protein levels in the hippocampus and frontal cortex followed by tests for learning and memory skills at 2, 5 and 10 months of age in eight groups of mice, i.e., RanBP9-629 single transgenic, APdE9 double transgenic, RanBP9-629/APdE9 triple transgenic, RanBP9-599 single transgenic, RanBP9-599/APdE9 triple transgenic, RanBP9-/- or RanBP9+/-, RanBP9-/- or RanBP9+/-/APdE9 and wild type litter-mate controls. Neuron Studio, software for automated spine density analysis, will be used to analyze dendritic branching points and spine numbers in Lucifer-yellow-stained pyramidal neurons after obtaining images by laser scanning confocal microscope. If RanBP9 is confirmed as a bona fide target in vivo in this study, the triple transgenic mice may prove to be useful as an accelerated model for synaptic and behavioral deficits.
Alzheimer?s disease is believed to be caused by loss of tiny junctions between neurons in the brain called synapses which positively correlates with the extent of memory loss. In spite of rigorous research efforts, how the damage to the synapses occurs is not known as of today. Our research objective is to identify the molecules responsible for synaptic damage so that future therapy for Alzheimer?s disease could be based on those molecules.
|Wang, Ruizhi; Wang, Hongjie; Carrera, Ivan et al. (2015) COPS5 protein overexpression increases amyloid plaque burden, decreases spinophilin-immunoreactive puncta, and exacerbates learning and memory deficits in the mouse brain. J Biol Chem 290:9299-309|
|Carrera, Iván; Etcheverría, Ignacio; Fernández-Novoa, Lucía et al. (2015) A comparative evaluation of a novel vaccine in APP/PS1 mouse models of Alzheimer's disease. Biomed Res Int 2015:807146|
|Wang, Ruizhi; Palavicini, Juan Pablo; Wang, Hongjie et al. (2014) RanBP9 overexpression accelerates loss of dendritic spines in a mouse model of Alzheimer's disease. Neurobiol Dis 69:169-79|
|Wang, Hongjie; Wang, Ruizhi; Xu, Shaohua et al. (2014) RanBP9 overexpression accelerates loss of pre and postsynaptic proteins in the AP?E9 transgenic mouse brain. PLoS One 9:e85484|
|Wang, Hongjie; Wang, Ruizhi; Lakshmana, Madepalli K et al. (2014) Substituted dithiazole piperazine benzamides as novel amyloid beta peptide reducing agents. Bioorg Med Chem Lett 24:4384-4388|
|Subramanian, Manikandan; Hayes, Crystal D; Thome, Joseph J et al. (2014) An AXL/LRP-1/RANBP9 complex mediates DC efferocytosis and antigen cross-presentation in vivo. J Clin Invest 124:1296-308|
|Palavicini, Juan Pablo; Wang, Hongjie; Minond, Dmitriy et al. (2014) RanBP9 overexpression down-regulates phospho-cofilin, causes early synaptic deficits and impaired learning, and accelerates accumulation of amyloid plaques in the mouse brain. J Alzheimers Dis 39:727-40|
|Wang, H; Lewsadder, M; Dorn, E et al. (2014) RanBP9 overexpression reduces dendritic arbor and spine density. Neuroscience 265:253-62|
|Wang, Hongjie; Dey, Debleena; Carrera, Ivan et al. (2013) COPS5 (Jab1) protein increases ? site processing of amyloid precursor protein and amyloid ? peptide generation by stabilizing RanBP9 protein levels. J Biol Chem 288:26668-77|
|Palavicini, J P; Wang, H; Bianchi, E et al. (2013) RanBP9 aggravates synaptic damage in the mouse brain and is inversely correlated to spinophilin levels in Alzheimer's brain synaptosomes. Cell Death Dis 4:e667|
Showing the most recent 10 out of 13 publications