Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI019635-14
Application #
2060976
Study Section
Microbial Physiology and Genetics Subcommittee 2 (MBC)
Project Start
1983-01-01
Project End
1997-11-30
Budget Start
1995-12-01
Budget End
1996-11-30
Support Year
14
Fiscal Year
1996
Total Cost
Indirect Cost
Name
University of California San Francisco
Department
Internal Medicine/Medicine
Type
Schools of Dentistry
DUNS #
073133571
City
San Francisco
State
CA
Country
United States
Zip Code
94143
Dombroski, A J (1997) Recognition of the -10 promoter sequence by a partial polypeptide of sigma70 in vitro. J Biol Chem 272:3487-94
Heisler, L M; Feng, G; Jin, D J et al. (1996) Amino acid substitutions in the two largest subunits of Escherichia coli RNA polymerase that suppress a defective Rho termination factor affect different parts of the transcription complex. J Biol Chem 271:14572-83
Dombroski, A J; Johnson, B D; Lonetto, M et al. (1996) The sigma subunit of Escherichia coli RNA polymerase senses promoter spacing. Proc Natl Acad Sci U S A 93:8858-62
Karls, R K; Jin, D J; Donohue, T J (1993) Transcription properties of RNA polymerase holoenzymes isolated from the purple nonsulfur bacterium Rhodobacter sphaeroides. J Bacteriol 175:7629-38
Heisler, L M; Suzuki, H; Landick, R et al. (1993) Four contiguous amino acids define the target for streptolydigin resistance in the beta subunit of Escherichia coli RNA polymerase. J Biol Chem 268:25369-75
Dombroski, A J; Walter, W A; Gross, C A (1993) Amino-terminal amino acids modulate sigma-factor DNA-binding activity. Genes Dev 7:2446-55
Lonetto, M; Gribskov, M; Gross, C A (1992) The sigma 70 family: sequence conservation and evolutionary relationships. J Bacteriol 174:3843-9
Dombroski, A J; Walter, W A; Record Jr, M T et al. (1992) Polypeptides containing highly conserved regions of transcription initiation factor sigma 70 exhibit specificity of binding to promoter DNA. Cell 70:501-12
Jin, D J; Gross, C A (1991) RpoB8, a rifampicin-resistant termination-proficient RNA polymerase, has an increased Km for purine nucleotides during transcription elongation. J Biol Chem 266:14478-85
Hager, D A; Jin, D J; Burgess, R R (1990) Use of Mono Q high-resolution ion-exchange chromatography to obtain highly pure and active Escherichia coli RNA polymerase. Biochemistry 29:7890-4

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