RNA editing in African trypanosomes is a post-transcriptional event which results in the formation of mitochondrial mRNAs differing from their genes by the insertion or deletion of uridylyl nucleotides.
In specific aim 1 the applicant proposes that RNA editing precedes by a enzyme cascade pathway initiated by the formatiom of a high molecular weight editing complex consisting of pre-edited mRNA, cognate guide RNA and associated proteins including an editing site specific endonuclease, a terminal uridylyl transferase and RNA ligase.
In aim 2 they propose to examine the function of an RNA editing complex associate protein (REAP-1).
In aim 3 they propose that RNA editing requires the assembly of a highly ordered complex consisting of RNAs and proteins.
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| Sykes, Steven; Szempruch, Anthony; Hajduk, Stephen (2015) The krebs cycle enzyme ?-ketoglutarate decarboxylase is an essential glycosomal protein in bloodstream African trypanosomes. Eukaryot Cell 14:206-15 |
| Sykes, Steven E; Hajduk, Stephen L (2013) Dual functions of ?-ketoglutarate dehydrogenase E2 in the Krebs cycle and mitochondrial DNA inheritance in Trypanosoma brucei. Eukaryot Cell 12:78-90 |
