Abstract

RNA editing in African trypanosomes is a post-transcriptional event which results in the formation of mitochondrial mRNAs differing from their genes by the insertion or deletion of uridylyl nucleotides.
In specific aim 1 the applicant proposes that RNA editing precedes by a enzyme cascade pathway initiated by the formatiom of a high molecular weight editing complex consisting of pre-edited mRNA, cognate guide RNA and associated proteins including an editing site specific endonuclease, a terminal uridylyl transferase and RNA ligase.
In aim 2 they propose to examine the function of an RNA editing complex associate protein (REAP-1).
In aim 3 they propose that RNA editing requires the assembly of a highly ordered complex consisting of RNAs and proteins.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI021401-16
Application #
6169920
Study Section
Tropical Medicine and Parasitology Study Section (TMP)
Program Officer
Fairfield, Alexandra
Project Start
1984-07-01
Project End
2003-05-31
Budget Start
2000-06-01
Budget End
2001-05-31
Support Year
16
Fiscal Year
2000
Total Cost
$226,959
Indirect Cost

  Institution

Name
University of Alabama Birmingham
Department
Biochemistry
Type
Schools of Medicine
DUNS #
004514360
City
Birmingham
State
AL
Country
United States
Zip Code
35294