The objectives of the proposed research are to define the genetic structure of natural populations of the human-pathogenic bacteria Legionella pneumophila and allied species, Neisseria gonorrhoeae, Haemophilus influenzae, and Escherichia coli. The method of genetic analysis involves determination of variation in multilocus genotypes, as indexed by the electrophoretic mobility of allelic variants of 20 enzymes in hundreds of individual bacterial isolates. Through application of the theory, laboratory methodology, and statistics of molecular population genetics, the proposed research seeks to develop powerful new genetic markers for studying the epidemiology and systematics of pathogenic bacteria. A major hypothesis being tested is that the genetic population structure of many human-pathogenic bacteria is basically clonal, with very limited chromosomal recombination, the pathogenic species being represented by relatively modest numbers of widely distributed clones carry special virulence factors, the genes of which are often in linkage disequilibrium with the enzyme loci being monitored. Variation in chromosomal genotype is analyzed in relation to serotype, biotype, and other phenotypic characters to assess the effectiveness of these characters in identifying strains in epidemiological and ecological research and to identify possible determinants of pathogenicity. In broad perspective, the proposed research seeks to establish for several pathogenic species of bacteria basic population genetic frameworks within which to organize data on the distribution of cell-surface antignes, toxins, adhesions, plasmids, and other characters that have been implicated as determinants of pathogenicity and disease specificity, while at the same time providing high-resolution marker systems for epidemiological, ecological, and other lines of research.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
1R01AI022144-01
Application #
3132880
Study Section
Microbial Physiology and Genetics Subcommittee 2 (MBC)
Project Start
1985-03-01
Project End
1989-02-28
Budget Start
1985-03-01
Budget End
1986-02-28
Support Year
1
Fiscal Year
1985
Total Cost
Indirect Cost
Name
University of Rochester
Department
Type
Schools of Arts and Sciences
DUNS #
208469486
City
Rochester
State
NY
Country
United States
Zip Code
14627
Eardly, B D; Nour, S M; van Berkum, P et al. (2005) Rhizobial 16S rRNA and dnaK genes: mosaicism and the uncertain phylogenetic placement of Rhizobium galegae. Appl Environ Microbiol 71:1328-35
Bumbaugh, Alyssa C; McGraw, Elizabeth A; Page, Kristen L et al. (2002) Sequence polymorphism of dotA and mip alleles mediating invasion and intracellular replication of Legionella pneumophila. Curr Microbiol 44:314-22
Davies, R L; Whittam, T S; Selander, R K (2001) Sequence diversity and molecular evolution of the leukotoxin (lktA) gene in bovine and ovine strains of Mannheimia (Pasteurella) haemolytica. J Bacteriol 183:1394-404
McGraw, E A; Li, J; Selander, R K et al. (1999) Molecular evolution and mosaic structure of alpha, beta, and gamma intimins of pathogenic Escherichia coli. Mol Biol Evol 16:12-22
Reid, S D; Selander, R K; Whittam, T S (1999) Sequence diversity of flagellin (fliC) alleles in pathogenic Escherichia coli. J Bacteriol 181:153-60
Beltran, P; Delgado, G; Navarro, A et al. (1999) Genetic diversity and population structure of Vibrio cholerae. J Clin Microbiol 37:581-90
Martin, C; Boyd, E F; Quentin, R et al. (1999) Enzyme polymorphism in Pseudomonas aeruginosa strains recovered from cystic fibrosis patients in France. Microbiology 145 ( Pt 9):2587-94
Davies, R L; Arkinsaw, S; Selander, R K (1997) Evolutionary genetics of Pasteurella haemolytica isolates recovered from cattle and sheep. Infect Immun 65:3585-93
Nelson, K; Wang, F S; Boyd, E F et al. (1997) Size and sequence polymorphism in the isocitrate dehydrogenase kinase/phosphatase gene (aceK) and flanking regions in Salmonella enterica and Escherichia coli. Genetics 147:1509-20
Boyd, E F; Li, J; Ochman, H et al. (1997) Comparative genetics of the inv-spa invasion gene complex of Salmonella enterica. J Bacteriol 179:1985-91

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