Salmonella is a common and important pathogen that kills millions of people each year, primarily children in developing countries. It was the first organism to be used as a bioterrorist weapon in the US and is listed as category B in the NIH list of BT agents (S. enterica as we describe here). The primary goal of my research is to use Salmonella as a model to understand how intracellular pathogens manipulate host cells to cause disease. Using our expertise with the Salmonella/macrophage model, coupled with genetics, molecular biology and transcriptional profiling, we will further our understanding of how intracellular pathogens regulate virulence gene expression. Our hypothesis is that multiple regulators respond to different cues within cells and that the signal becomes integrated, perhaps by one or a few master regulators, to express specific subsets of virulence factors required for survival and growth within different cells and tissues of the host. Salmonella is a model for studying intracellular pathogenesis without equal because of its established genetics and simple and inexpensive animal model - the mouse. Understanding how Salmonella survives and replicates within the host and how it expresses virulence genes at the appropriate time and place during infection will identify new therapeutic targets and provide a paradigm for understanding other pathogens.

Public Health Relevance

Salmonella is a common and important pathogen that kills millions of people each year, primarily children in developing countries. We wish to understand how Salmonella survives within and manipulates host cells for its own benefit. The goal of this research is to discover how Salmonella expresses virulence genes at the appropriate time and place during the infection in order to identify new therapeutic targets and to provide a paradigm for understanding other intracellular pathogen.

National Institute of Health (NIH)
National Institute of Allergy and Infectious Diseases (NIAID)
Research Project (R01)
Project #
Application #
Study Section
Special Emphasis Panel (ZRG1-BACP-H (02))
Program Officer
Alexander, William A
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Oregon Health and Science University
Schools of Medicine
United States
Zip Code
Ansong, Charles; Deatherage, Brooke L; Hyduke, Daniel et al. (2013) Studying salmonellae and yersiniae host-pathogen interactions using integrated 'omics and modeling. Curr Top Microbiol Immunol 363:21-41
Hovis, Kelley M; Mojica, Sergio; McDermott, Jason E et al. (2013) Genus-optimized strategy for the identification of chlamydial type III secretion substrates. Pathog Dis 69:213-22
Kidwai, Afshan S; Mushamiri, Ivy; Niemann, George S et al. (2013) Diverse secreted effectors are required for Salmonella persistence in a mouse infection model. PLoS One 8:e70753
Niemann, George S; Brown, Roslyn N; Mushamiri, Ivy T et al. (2013) RNA type III secretion signals that require Hfq. J Bacteriol 195:2119-25
Yoon, Hyunjin; Gros, Phillipe; Heffron, Fred (2011) Quantitative PCR-based competitive index for high-throughput screening of Salmonella virulence factors. Infect Immun 79:360-8
Yoon, Hyunjin; Ansong, Charles; Adkins, Joshua N et al. (2011) Discovery of Salmonella virulence factors translocated via outer membrane vesicles to murine macrophages. Infect Immun 79:2182-92
McDermott, Jason E; Corrigan, Abigail; Peterson, Elena et al. (2011) Computational prediction of type III and IV secreted effectors in gram-negative bacteria. Infect Immun 79:23-32
Niemann, George S; Brown, Roslyn N; Gustin, Jean K et al. (2011) Discovery of novel secreted virulence factors from Salmonella enterica serovar Typhimurium by proteomic analysis of culture supernatants. Infect Immun 79:33-43
Ansong, Charles; Tolic, Nikola; Purvine, Samuel O et al. (2011) Experimental annotation of post-translational features and translated coding regions in the pathogen Salmonella Typhimurium. BMC Genomics 12:433
McDermott, Jason E; Yoon, Hyunjin; Nakayasu, Ernesto S et al. (2011) Technologies and approaches to elucidate and model the virulence program of salmonella. Front Microbiol 2:121

Showing the most recent 10 out of 50 publications