Abstract

This proposal is based on the hypothesis that African trypanosomes utilize a variety of signaling pathways to coordinate life cycle events. During the course of studies on the control of Ca+ currents in T. brucei, they discoved key components of an eicosanoic acid pathway. The present proposal will examine how trypanosomes regulate Ca+/eicosanoic acid signals.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI024627-11
Application #
6170296
Study Section
Tropical Medicine and Parasitology Study Section (TMP)
Program Officer
Fairfield, Alexandra
Project Start
1987-03-01
Project End
2003-05-31
Budget Start
2000-06-01
Budget End
2001-05-31
Support Year
11
Fiscal Year
2000
Total Cost
$189,203
Indirect Cost

  Institution

Name
Southern Methodist University
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
City
Dallas
State
TX
Country
United States
Zip Code
75205

  Publications

Ridgley, E L; Ruben, L (2001) Phospholipase from Trypanosoma brucei releases arachidonic acid by sequential sn-1, sn-2 deacylation of phospholipids. Mol Biochem Parasitol 114:29-40
Ridgley, E; Webster, P; Patton, C et al. (2000) Calmodulin-binding properties of the paraflagellar rod complex from Trypanosoma brucei. Mol Biochem Parasitol 109:195-201
Parsons, M; Ruben, L (2000) Pathways involved in environmental sensing in trypanosomatids. Parasitol Today 16:56-62
Ridgley, E L; Xiong, Z H; Ruben, L (1999) Reactive oxygen species activate a Ca2+-dependent cell death pathway in the unicellular organism Trypanosoma brucei brucei. Biochem J 340 ( Pt 1):33-40
Xiong, Z H; Ruben, L (1998) Trypanosoma brucei: the dynamics of calcium movement between the cytosol, nucleus, and mitochondrion of intact cells. Exp Parasitol 88:231-9
Eintracht, J; Maathai, R; Mellors, A et al. (1998) Calcium entry in Trypanosoma brucei is regulated by phospholipase A2 and arachidonic acid. Biochem J 336 ( Pt 3):659-66
Xiong, Z H; Ridgley, E L; Enis, D et al. (1997) Selective transfer of calcium from an acidic compartment to the mitochondrion of Trypanosoma brucei. Measurements with targeted aequorins. J Biol Chem 272:31022-8
Ruben, L; Akins, C D; Haghighat, N G et al. (1996) Calcium influx in Trypanosoma brucei can be induced by amphiphilic peptides and amines. Mol Biochem Parasitol 81:191-200
Ridgley, E L; Xiong, Z H; Kaur, K J et al. (1996) Genomic organization and expression of elongation factor-1 alpha genes in Trypanosoma brucei. Mol Biochem Parasitol 79:119-23
Xiong, Z H; Ruben, L (1996) Nuclear calcium flux in Trypanosoma brucei can be quantified with targeted aequorin. Mol Biochem Parasitol 83:57-67

Showing the most recent 10 out of 17 publications