Treponema pallidum (Tp), the causative agent of syphilis, is one of most poorly understood of all bacterial pathogens. That Tp cannot be cultivated continuously in vitro has severely hampered progress towards understanding many of the complexities associated with Tp membrane biology and syphilis pathogenesis. Tp encodes a large number of membrane lipoproteins (LPs) that have widespread importance. Over the past funding interval, we have pursued a "structural biology" approach for characterizing the Tp LPs by solving their three-dimensional structures and then testing hypotheses about their possible functions. We now have compelling evidence that this unconventional approach represents a successful discovery platform. First, we have described in Tp a periplasmic transport system comprised of two LPs (TP0956/TP0957) that make up a TPR-protein-associated TRAP transport system. This system has many molecular features never before described for a bacterial transport apparatus. TP0956/TP0957 forms a complex that spans precisely the width of the Tp periplasm, and which contains a hydrophobic pore;we shall attempt to determine the ligand carried by this transport complex (Aim 3). We also have identified an essential ABC-type of riboflavin transporter (RfuABCD) in pathogenic spirochetes. This type of riboflavin transport system has never been previously described in bacteria, but it has prompted a re-examination of the potential importance of flavin-containing cofactors and flavoproteins to Tp biology. We also have discovered in Tp a novel FAD pyrophosphatase (TP0796/Ftp), the first such recognition of this type of enzymatic activity in any bacterium;the discovery has further implications for flavin utilization/homeostasis in Tp and, along with our other findings, engenders a new conception that Tp has evolved a "flavin-centric" lifestyle. Indeed, we have found that Tp encodes a homolog of RnfD (TP0151) that, in other bacteria, requires FMN as a co-factor and typically is a member of an RnfABCDGE operon for redox-driven ion pumps. Tp has been thought NOT to encode such as system, but we also have found a homolog of RnfC (TP0152) (within the gene cluster tp0147-tp0153). These combined findings engender strong supposition that Tp encodes a complete Rnf redox system, something that challenges existing dogma. Establishing that one or more of these Rnf-like proteins interact with each other will provide further evidence of an active Rnf-like redox system in Tp (Aim 2). A better understanding of this "flavin-centric" lifestyle for Tp may result in valuable new intervention strategies that target either riboflavin uptake or flavin utilization pathways, and could also provide further nutritional information leading to the eventual in vitro cultivation of Tp (Aim 1). Finally, all of our discoveries underscore the power of our "structure-to-function" approach, and thus we anticipate many more new advancements from discerning the structures of the Tp LPs (Aim 4.).
Syphilis, caused by the bacterium Treponema pallidum, continues to play prominently as a sexually transmitted infection in the United States and worldwide. The membrane lipoproteins of T. pallidum are recognized as being particularly vital to the biology of the spirochete. Given their importance, we are targeting these molecules for strategic study. Discerning the functions of T. pallidum's membrane lipoproteins can potentially help to explain many of the salient features of T. pallidum's peculiar membrane biology, elucidate key aspects of the spirochete's parasitic strategy, prompt new modalities to thwart the infectious process, and spawn new avenues of investigation for potentially designing novel antimicrobial drugs.
|Brautigam, Chad A; Deka, Ranjit K; Norgard, Michael V (2013) Purification, crystallization and preliminary X-ray analysis of TP0435 (Tp17) from the syphilis spirochete Treponema pallidum. Acta Crystallogr Sect F Struct Biol Cryst Commun 69:453-5|
|Zhao, Huaying; Brautigam, Chad A; Ghirlando, Rodolfo et al. (2013) Overview of current methods in sedimentation velocity and sedimentation equilibrium analytical ultracentrifugation. Curr Protoc Protein Sci Chapter 20:Unit20.12|
|Deka, Ranjit K; Brautigam, Chad A; Liu, Wei Z et al. (2013) The TP0796 lipoprotein of Treponema pallidum is a bimetal-dependent FAD pyrophosphatase with a potential role in flavin homeostasis. J Biol Chem 288:11106-21|
|Deka, Ranjit K; Brautigam, Chad A; Biddy, Brent A et al. (2013) Evidence for an ABC-type riboflavin transporter system in pathogenic spirochetes. MBio 4:e00615-12|
|Deka, Ranjit K; Brautigam, Chad A; Goldberg, Martin et al. (2012) Structural, bioinformatic, and in vivo analyses of two Treponema pallidum lipoproteins reveal a unique TRAP transporter. J Mol Biol 416:678-96|
|Brautigam, Chad A; Deka, Ranjit K; Schuck, Peter et al. (2012) Structural and thermodynamic characterization of the interaction between two periplasmic Treponema pallidum lipoproteins that are components of a TPR-protein-associated TRAP transporter (TPAT). J Mol Biol 420:70-86|
|Padrick, Shae B; Deka, Ranjit K; Chuang, Jacinta L et al. (2010) Determination of protein complex stoichiometry through multisignal sedimentation velocity experiments. Anal Biochem 407:89-103|
|Machius, Mischa; Brautigam, Chad A; Tomchick, Diana R et al. (2007) Structural and biochemical basis for polyamine binding to the Tp0655 lipoprotein of Treponema pallidum: putative role for Tp0655 (TpPotD) as a polyamine receptor. J Mol Biol 373:681-94|
|Deka, Ranjit K; Brautigam, Chad A; Tomson, Farol L et al. (2007) Crystal structure of the Tp34 (TP0971) lipoprotein of treponema pallidum: implications of its metal-bound state and affinity for human lactoferrin. J Biol Chem 282:5944-58|
|Deka, Ranjit K; Brautigam, Chad A; Yang, Xiaofeng F et al. (2006) The PnrA (Tp0319; TmpC) lipoprotein represents a new family of bacterial purine nucleoside receptor encoded within an ATP-binding cassette (ABC)-like operon in Treponema pallidum. J Biol Chem 281:8072-81|
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