PU.1 is an ETS family transcription factor that is crucial for the development of multiple hematopoietic lineages including macrophages, B cells, mast cells and neutrophils. In the previous grant period we demonstrated that PU.1 is also expressed in T cells, specifically in Th2 cells. PU.1 expression segregates into Th2 populations secreting low levels of IL-4, but promotes the expression of chemokines associated with allergic inflammation. PU.1-deficient Th2 cells have increased Th2 cytokine production and increased homogeneity of cytokine secretion, in that there are more cells secreting two or more cytokines. Thus, PU.1 regulates heterogeneity in Th2 populations. In the last granting period we have also demonstrated that PU.1 is required for IL-9 expression in the recently described Th9 population of Th cells in both mouse and human cultures, and that PU.1 is expressed at higher levels in Th9 than in Th2 cells. We further show that the development of allergic inflammation in mice that have a T cell specific deletion of PU.1 is decreased, correlating with decreased expression of IL-9 and Th2 chemokines. This demonstrates a requirement for PU.1 in the development of allergic inflammation, a process that was thought to depend largely on Th2 cells. In this proposal, we will further explore the role of PU.1 in Th9 cells, both in the context of gene regulation of IL-9, the development of the Th9 phenotype, and the requirement for PU.1-dependent Th9 cells in the development of allergic inflammation. Our hypothesis for this proposal is that PU.1 is an important regulator of type 2 inflammation and that PU.1-depnedent regulation of the Th2/Th9 phenotypes is a critical component of developing allergic inflammation.
Our Aims for this proposal are 1. Define the requirement for PU.1 in mediating allergic inflammation in induced and spontaneous model systems;2. Determine the structural and functional requirements for the ability of PU.1 to regulate IL-9;and 3. Define the requirement for PU.1 in plasticity and stability of the IL-9-secreting T cell phenotype. Our overall goal for this application is to define the role of Th9 cells in allergic inflammation, and the role of PU.1 in directing this phenotype. The information learned from these studies will provide a greater understanding of the role this subset plays in allergic inflammation, and how targeting this subset, or its functions, might be developed as therapy for allergic disease in humans.
For many years Th2 cells were the only T cells thought to control allergic inflammation. We present preliminary data that a newly described subset of cells called Th9 cells, are also required for allergic inflammation and in this proposal we investigate factors that control their development and function. Results from these studies will provide new information about how allergic disease develops, and how T cells could be targeted for the treatment of allergic disease.
|Olson, Matthew R; Verdan, Felipe Fortino; Hufford, Matthew M et al. (2016) STAT3 Impairs STAT5 Activation in the Development of IL-9-Secreting T Cells. J Immunol 196:3297-304|
|Kaplan, Mark H (2016) The transcription factor network in Th9 cells. Semin Immunopathol :|
|Koh, Byunghee; Hufford, Matthew M; Pham, Duy et al. (2016) The ETS Family Transcription Factors Etv5 and PU.1 Function in Parallel To Promote Th9 Cell Development. J Immunol 197:2465-72|
|Ebel, Mark E; Awe, Olufolakemi; Kaplan, Mark H et al. (2015) Diverse inflammatory cytokines induce selectin ligand expression on murine CD4 T cells via p38Î± MAPK. J Immunol 194:5781-8|
|Awe, Olufolakemi; Hufford, Matthew M; Wu, Hao et al. (2015) PU.1 Expression in T Follicular Helper Cells Limits CD40L-Dependent Germinal Center B Cell Development. J Immunol 195:3705-15|
|Sehra, Sarita; Yao, Weiguo; Nguyen, Evelyn T et al. (2015) TH9 cells are required for tissue mast cell accumulation during allergic inflammation. J Allergy Clin Immunol 136:433-40.e1|
|Chang, Daniel; Yao, Weiguo; Tiller, Christina J et al. (2015) Exhaled nitric oxide during infancy as a risk factor for asthma and airway hyperreactivity. Eur Respir J 45:98-106|
|Richard, Arianne C; Tan, Cuiyan; Hawley, Eric T et al. (2015) The TNF-family ligand TL1A and its receptor DR3 promote T cell-mediated allergic immunopathology by enhancing differentiation and pathogenicity of IL-9-producing T cells. J Immunol 194:3567-82|
|Kaplan, Mark H; Hufford, Matthew M; Olson, Matthew R (2015) The development and in vivo function of T helper 9 cells. Nat Rev Immunol 15:295-307|
|Sarria, Edgar E; Mattiello, Rita; Yao, Weiguo et al. (2014) Atopy, cytokine production, and airway reactivity as predictors of pre-school asthma and airway responsiveness. Pediatr Pulmonol 49:132-9|
Showing the most recent 10 out of 37 publications