Acinetobacter baumannii has emerged from an organism of questionable pathogenicity to an infectious agent of importance to hospitals worldwide causing increased patient morbidity and mortality. The reasons for the emergence of A. baumannii are unknown and no interventions have been successful at curbing this emergence. For these reasons, A. baumannii is a significant public health problem. To our knowledge, no cohort similar to the one described in this application has been created to investigate the epidemiology of A. baumannii. This knowledge is essential in order to develop and implement new, cost-effective infection control strategies aimed at reducing nosocomial infection and the worldwide spread of A. baumannii. The long-term objective of this renewal application is to continue to understand the epidemiology of emergent Gram- negative bacteria and antibiotic-resistant bacteria with a focus in this application on A. baumannii.
The aims of this renewal are:
Aim 1) Construct a prospective cohort of intensive care unit (ICU) patients to quantify the proportion of A. baumannii acquisition that is attributable to patient-to-patient transmission.
Aim 2) Culture several environmental sites in the hospital rooms of patients colonized and infected with A. baumannii to determine sites of frequent contamination and determine whether terminal cleaning between patients is effective.
Aim 3) Identify potential genetic factors that contribute to high transmissibility of A. baumannii using genomic sequencing and comparative genomic techniques. This application is a competitive renewal of an ongoing ICU cohort. The cohort consists of patients admitted to the ICU who have peri-rectal surveillance cultures obtained. The peri-rectal cultures can be used to analyze in a retrospective fashion many bacterial species. The peri-rectal cultures are linked to demographic, comorbidity, laboratory, and medication data that are contained in a relational database. Thus the cohort serves as a rich mechanism of understanding the epidemiology of a number of different emerging bacteria and hospital pathogens.

Public Health Relevance

Acinetobacter baumannii has emerged from an organism of questionable pathogenicity to an infectious agent of importance to hospitals worldwide causing increased patient morbidity and mortality. The reasons for the emergence of A. baumannii are unknown and no interventions have been successful at curbing this emergence. For these reasons, A. baumannii is a significant public health problem.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
2R01AI060859-05A1
Application #
7782458
Study Section
Infectious Diseases, Reproductive Health, Asthma and Pulmonary Conditions Study Section (IRAP)
Program Officer
Korpela, Jukka K
Project Start
2004-07-01
Project End
2014-05-31
Budget Start
2010-06-01
Budget End
2011-05-31
Support Year
5
Fiscal Year
2010
Total Cost
$180,891
Indirect Cost
Name
University of Maryland Baltimore
Department
Public Health & Prev Medicine
Type
Schools of Medicine
DUNS #
188435911
City
Baltimore
State
MD
Country
United States
Zip Code
21201
Johnson, J Kristie; Robinson, Gwen L; Zhao, LiCheng et al. (2016) Comparison of molecular typing methods for the analyses of Acinetobacter baumannii from ICU patients. Diagn Microbiol Infect Dis 86:345-350
Johnson, J Kristie; Wilson, Lucy E; Zhao, Licheng et al. (2014) Point prevalence of Klebsiella pneumoniae carbapenemase-producing Enterobacteriaceae in Maryland. Infect Control Hosp Epidemiol 35:443-5
Hazen, Tracy H; Zhao, LiCheng; Boutin, Mallory A et al. (2014) Comparative genomics of an IncA/C multidrug resistance plasmid from Escherichia coli and Klebsiella isolates from intensive care unit patients and the utility of whole-genome sequencing in health care settings. Antimicrob Agents Chemother 58:4814-25
Hazen, Tracy H; Zhao, LiCheng; Sahl, Jason W et al. (2014) Characterization of Klebsiella sp. strain 10982, a colonizer of humans that contains novel antibiotic resistance alleles and exhibits genetic similarities to plant and clinical Klebsiella isolates. Antimicrob Agents Chemother 58:1879-88
Ajao, Adebola O; Johnson, J Kristie; Harris, Anthony D et al. (2013) Risk of acquiring extended-spectrum ?-lactamase-producing Klebsiella species and Escherichia coli from prior room occupants in the intensive care unit. Infect Control Hosp Epidemiol 34:453-8
Strassle, Paula; Thom, Kerri A; Johnson, J Kristie et al. (2012) The effect of terminal cleaning on environmental contamination rates of multidrug-resistant Acinetobacter baumannii. Am J Infect Control 40:1005-7
Thom, Kerri A; Maragakis, Lisa L; Richards, Katie et al. (2012) Assessing the burden of Acinetobacter baumannii in Maryland: a statewide cross-sectional period prevalence survey. Infect Control Hosp Epidemiol 33:883-8
Ranke, Tarah D; Strassle, Paula; Harris, Anthony D et al. (2012) Recovery of Gram-negative bacilli in stored endotracheal aspirates. J Clin Microbiol 50:2791-2
Thom, Kerri A; Howard, Tracy; Sembajwe, Sophie et al. (2012) Comparison of swab and sponge methodologies for identification of Acinetobacter baumannii from the hospital environment. J Clin Microbiol 50:2140-1
Hazen, Tracy H; Robinson, Gwen L; Harris, Anthony D et al. (2012) Genome sequence of Klebsiella oxytoca 11492-1, a nosocomial isolate possessing a FOX-5 AmpC ýý-lactamase. J Bacteriol 194:3028-9

Showing the most recent 10 out of 40 publications