This revised supplement is responsive to the notice NOT-OD-09-058 entitled: """"""""Enabling RPGs to Leverage NCRR Center and Center-like Programs"""""""".
The aims of this revision are designed to utilize the existing framework of our ongoing R01 entitled: """"""""S. aureus transmission in Northern Manhattan."""""""" The overall goal is to define the importance of strain ST398 as an emerging pathogen in our community and to determine the basis for its virulence. Our new aims are listed below. a. What are the reservoirs and modes of transmission of ST398 in Northern Manhattan? A cluster-based study design will be used to define the basis for ST398 transmission in Northern Manhattan. Subjects either colonized or infected with ST398 will be identified, interviewed, cultured. All contacts and their secondary contacts will be identified and asked to participate. Factors associated with acquisition and transmission of this strain will be determined. Efforts will be made to enroll all network members including recent contacts in the Dominican Republic. In light of the past history of an animal origin for ST398 we will examine potential animal reservoirs in the community including pet stores, veterinarians, butchers and pet groomers. Spatial mapping of the location the strains were identified will also be used to identify potential patterns of or reservoirs for transmission. b. What genetic alterations are responsible for the adaptation of strain ST398 to person-to-person transmission? We will use total genome sequencing to compare the Northern Manhattan ST398 isolates with those isolates that have been directly associated with animal related infections. We will identify regions of the ST398 genome that are unique and might explain the increased transmissibility and virulence of these clones. A molecular beacon assay will be developed to more easily identify ST398. This will allow more efficient and rapid screening for ST398 in large collections of S. aureus isolates. The study will utilize the resources of the Columbia University Irving Center for Clinical and Translational Research to help carry out this community-based investigation. The revision will provide two new positions as well as provide additional work for the total genome sequencing company assisting with aim 2.

Public Health Relevance

The goal of this revised supplement is to define the importance of S. aureus strain ST398, a strain originally associated with infections among animal workers now prevalent in Northern Manhattan, as an emerging pathogen in our community and to determine the basis for its virulence.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
3R01AI077690-02S1
Application #
7892905
Study Section
Special Emphasis Panel (ZRG1-PSE-A (95))
Program Officer
Huntley, Clayton C
Project Start
2008-04-01
Project End
2012-05-31
Budget Start
2010-06-01
Budget End
2012-05-31
Support Year
2
Fiscal Year
2010
Total Cost
$339,002
Indirect Cost
Name
Columbia University (N.Y.)
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
621889815
City
New York
State
NY
Country
United States
Zip Code
10032
Uhlemann, Anne-Catrin; McAdam, Paul R; Sullivan, Sean B et al. (2017) Evolutionary Dynamics of Pandemic Methicillin-Sensitive Staphylococcus aureus ST398 and Its International Spread via Routes of Human Migration. MBio 8:
Knox, Justin; Sullivan, Sean B; Urena, Julia et al. (2016) Association of Environmental Contamination in the Home With the Risk for Recurrent Community-Associated, Methicillin-Resistant Staphylococcus aureus Infection. JAMA Intern Med 176:807-15
Knox, Justin; Uhlemann, Anne-Catrin; Lowy, Franklin D (2015) Staphylococcus aureus infections: transmission within households and the community. Trends Microbiol 23:437-44
Knox, J; Van Rijen, M; Uhlemann, A-C et al. (2015) Community-associated methicillin-resistant Staphylococcus aureus transmission in households of infected cases: a pooled analysis of primary data from three studies across international settings. Epidemiol Infect 143:354-65
Uhlemann, Anne-Catrin; Lowy, Franklin D (2014) Reply to Gandra et al. Clin Infect Dis 58:307-8
Uhlemann, Anne-Catrin; Otto, Michael; Lowy, Franklin D et al. (2014) Evolution of community- and healthcare-associated methicillin-resistant Staphylococcus aureus. Infect Genet Evol 21:563-74
Uhlemann, Anne-Catrin; Hafer, Cory; Miko, Benjamin A et al. (2013) Emergence of sequence type 398 as a community- and healthcare-associated methicillin-susceptible staphylococcus aureus in northern Manhattan. Clin Infect Dis 57:700-3
David, Michael Z; Siegel, Jane; Lowy, Franklin D et al. (2013) Asymptomatic carriage of sequence type 398, spa type t571 methicillin-susceptible Staphylococcus aureus in an urban jail: a newly emerging, transmissible pathogenic strain. J Clin Microbiol 51:2443-7
Knox, Justin; Uhlemann, Anne-Catrin; Miller, Maureen et al. (2012) Environmental contamination as a risk factor for intra-household Staphylococcus aureus transmission. PLoS One 7:e49900
Uhlemann, Anne-Catrin; Kennedy, Adam D; Martens, Craig et al. (2012) Toward an understanding of the evolution of Staphylococcus aureus strain USA300 during colonization in community households. Genome Biol Evol 4:1275-85

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