New Targets for Reproductive Control of Mosquito Vectors. In this project, we will investigate the male seminal fluid proteins (SFPs) of two major disease vectors of dengue and Chikungunya viruses: the yellow fever mosquito (Aedes aegypti) and the Asian tiger mosquito (Aedes albopictus). Both dengue and Chikungunya are increasing in incidence globally and there is an immediate need to develop novel and effective vector control strategies for these two species. In this study we will conduct two specific aims to identify and functionally characterize SFPs, for the first time, in Ae. aegypti and Ae. albopictus. Our goal is to directly identify new mosquito SFP targets that could be manipulated as a novel means of reproductive control, or to modify the blood feeding physiology of insects that transmit deadly pathogens, with the long-term goal to reduce or eliminate virus transmission to people.
In this study, we will directly identify and determine the function of seminal fluid proteins produced in male mosquitoes and transferred to females during mating. We will identify targets that could be manipulated as a novel means of reproductive control or as a means of modifying the blood feeding physiology of insects that transmit deadly pathogens causing diseases such as dengue and Chikungunya, with the long-term goal to reduce or eliminate virus transmission to people.
|Alfonso-Parra, Catalina; Avila, Frank W; Deewatthanawong, Prasit et al. (2014) Synthesis, depletion and cell-type expression of a protein from the male accessory glands of the dengue vector mosquito Aedes aegypti. J Insect Physiol 70:117-24|
|Perry, Jennifer C; Sirot, Laura; Wigby, Stuart (2013) The seminal symphony: how to compose an ejaculate. Trends Ecol Evol 28:414-22|
|Helinski, Michelle E H; Valerio, Laura; Facchinelli, Luca et al. (2012) Evidence of polyandry for Aedes aegypti in semifield enclosures. Am J Trop Med Hyg 86:635-41|