New Targets for Reproductive Control of Mosquito Vectors. In this project, we will investigate the male seminal fluid proteins (SFPs) of two major disease vectors of dengue and Chikungunya viruses: the yellow fever mosquito (Aedes aegypti) and the Asian tiger mosquito (Aedes albopictus). Both dengue and Chikungunya are increasing in incidence globally and there is an immediate need to develop novel and effective vector control strategies for these two species. In this study we will conduct two specific aims to identify and functionally characterize SFPs, for the first time, in Ae. aegypti and Ae. albopictus. Our goal is to directly identify new mosquito SFP targets that could be manipulated as a novel means of reproductive control, or to modify the blood feeding physiology of insects that transmit deadly pathogens, with the long-term goal to reduce or eliminate virus transmission to people.
In this study, we will directly identify and determine the function of seminal fluid proteins produced in male mosquitoes and transferred to females during mating. We will identify targets that could be manipulated as a novel means of reproductive control or as a means of modifying the blood feeding physiology of insects that transmit deadly pathogens causing diseases such as dengue and Chikungunya, with the long-term goal to reduce or eliminate virus transmission to people.
|Degner, Ethan C; Harrington, Laura C (2016) A mosquito sperm's journey from male ejaculate to egg: Mechanisms, molecules, and methods for exploration. Mol Reprod Dev 83:897-911|
|Alfonso-Parra, Catalina; Ahmed-Braimah, Yasir H; Degner, Ethan C et al. (2016) Mating-Induced Transcriptome Changes in the Reproductive Tract of Female Aedes aegypti. PLoS Negl Trop Dis 10:e0004451|
|Degner, Ethan C; Harrington, Laura C (2016) Polyandry Depends on Postmating Time Interval in the Dengue Vector Aedes aegypti. Am J Trop Med Hyg 94:780-5|
|Sirot, Laura K; Wong, Alex; Chapman, Tracey et al. (2015) Sexual conflict and seminal fluid proteins: a dynamic landscape of sexual interactions. Cold Spring Harb Perspect Biol 7:a017533|
|Alfonso-Parra, Catalina; Avila, Frank W; Deewatthanawong, Prasit et al. (2014) Synthesis, depletion and cell-type expression of a protein from the male accessory glands of the dengue vector mosquito Aedes aegypti. J Insect Physiol 70:117-24|
|Boes, Kathryn E; Ribeiro, JosÃ© M C; Wong, Alex et al. (2014) Identification and characterization of seminal fluid proteins in the Asian tiger mosquito, Aedes albopictus. PLoS Negl Trop Dis 8:e2946|
|Perry, Jennifer C; Sirot, Laura; Wigby, Stuart (2013) The seminal symphony: how to compose an ejaculate. Trends Ecol Evol 28:414-22|
|Helinski, Michelle E H; Deewatthanawong, Prasit; Sirot, Laura K et al. (2012) Duration and dose-dependency of female sexual receptivity responses to seminal fluid proteins in Aedes albopictus and Ae. aegypti mosquitoes. J Insect Physiol 58:1307-13|
|Helinski, Michelle E H; Harrington, Laura C (2012) The role of male harassment on female fitness for the dengue vector mosquito Aedes aegypti. Behav Ecol Sociobiol 66:1131-1140|
|Helinski, Michelle E H; Valerio, Laura; Facchinelli, Luca et al. (2012) Evidence of polyandry for Aedes aegypti in semifield enclosures. Am J Trop Med Hyg 86:635-41|
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