As bacteria grow and divide, they constantly break down peptidoglycan strands to allow for cell wall expansion or reshaping the cell wall for septation and cell separation. Unlike most Gram-negative bacteria, Neisseria gonorrhoeae releases significant amounts of the peptidoglycan fragments produced during these processes. The released peptidoglycan fragments induce inflammatory responses in multiple cell types and cause sloughing of ciliated cells in the Fallopian tube. The ciliated cell sloughing is characteristic of gonococcal pelvic inflammatory disease and leads to the loss of the ability to move an egg down the oviduct. We have worked to characterize the enzymes that act in peptidoglycan breakdown so that we can understand the process of peptidoglycan fragment production. We are characterizing these enzymes to determine their substrates and products as well as subcellular localization and location relative to the septum on the gonococcal cell. Using mutants lacking genes for certain peptidoglycan degradation enzymes, we determined that ciliated cell sloughing occurs in response to peptidoglycan monomers with a three amino acid peptide, implicating pattern-recognition receptor NOD1 in the cellular response. We also discovered that gonococci release a second immunostimulatory muropeptide, a glycosidically-linked peptidoglycan dimer that stimulates NOD2. In this study we will characterize lytic transglycosylases for the generation of the peptidoglycan dimers and characterize the enzymes that create the free peptide that serves as a NOD1 agonist. We will examine AmpG function in peptidoglycan fragment recycling and determine how gonococci sense peptidoglycan fragments in the cytoplasm and regulate peptidoglycan fragment release or uptake. Finally we will characterize cellular responses to released peptidoglycan fragments to understand how Fallopian tube tissue is damaged in pelvic inflammatory disease and how neutrophils respond to released eptidoglycan fragments.

Public Health Relevance

As bacteria grow, they release small fragments of the bacterial cell wall. The organism that causes gonorrhea and pelvic inflammatory disease releases more of these cell wall fragments than most other bacteria and elicits an immune response that is damaging to human tissue. These studies will determine the mechanisms involved in producing the damaging cell wall fragments and characterize the human cell responses that result in inflammation and tissue destruction.

National Institute of Health (NIH)
National Institute of Allergy and Infectious Diseases (NIAID)
Research Project (R01)
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Special Emphasis Panel (ZRG1)
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Vincent, Leah Rebecca
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University of Wisconsin Madison
Schools of Medicine
United States
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Schaub, Ryan E; Dillard, Joseph P (2017) Digestion of Peptidoglycan and Analysis of Soluble Fragments. Bio Protoc 7:
Ragland, Stephanie A; Schaub, Ryan E; Hackett, Kathleen T et al. (2017) Two lytic transglycosylases in Neisseria gonorrhoeae impart resistance to killing by lysozyme and human neutrophils. Cell Microbiol 19:
Lenz, Jonathan D; Hackett, Kathleen T; Dillard, Joseph P (2017) A Single Dual-Function Enzyme Controls the Production of Inflammatory NOD Agonist Peptidoglycan Fragments by Neisseria gonorrhoeae. MBio 8:
Lenz, Jonathan D; Stohl, Elizabeth A; Robertson, Rosanna M et al. (2016) Amidase Activity of AmiC Controls Cell Separation and Stem Peptide Release and Is Enhanced by NlpD in Neisseria gonorrhoeae. J Biol Chem 291:10916-33
Bhoopalan, Senthil V; Piekarowicz, Andrzej; Lenz, Jonathan D et al. (2016) nagZ Triggers Gonococcal Biofilm Disassembly. Sci Rep 6:22372
Schaub, Ryan E; Chan, Yolande A; Lee, Mijoon et al. (2016) Lytic transglycosylases LtgA and LtgD perform distinct roles in remodeling, recycling and releasing peptidoglycan in Neisseria gonorrhoeae. Mol Microbiol 102:865-881
Krasity, Benjamin C; Troll, Joshua V; Lehnert, Erik M et al. (2015) Structural and functional features of a developmentally regulated lipopolysaccharide-binding protein. MBio 6:e01193-15
Stohl, Elizabeth A; Lenz, Jonathan D; Dillard, Joseph P et al. (2015) The Gonococcal NlpD Protein Facilitates Cell Separation by Activating Peptidoglycan Cleavage by AmiC. J Bacteriol 198:615-22
Woodhams, Katelynn L; Chan, Jia Mun; Lenz, Jonathan D et al. (2013) Peptidoglycan fragment release from Neisseria meningitidis. Infect Immun 81:3490-8