This project focuses on the outdoor resting and sugar-feeding behaviors of Anopheles malaria vector populations in Mali, West Africa, where, as in most African countries, malaria is a serious public health problem. An understanding of mosquito behaviors in the outdoor environment is becoming increasingly important because there is a growing awareness that vector control tools beyond those used exclusively inside houses are needed to achieve successful malaria control and local elimination. Currently, for the major malaria vector species in Africa, Anopheles gambiae, An. arabiensis, and An. funestus, there is only limited knowledge on their outdoor resting behavior, and the role of sugar or nectars in their life history is mostly speculated due to a lack of field-based evidence. Furthermore, resting and sugar feeding behaviors likely exhibit extensive phenotypic plasticity but the role of the ecological environment as the possible key driver has not been investigated. Therefore, the goal of this project is to investigate Anopheles mosquito outdoor resting and sugar- feeding behaviors at ecologically diverse sites in Mali to determine how complex relationships between these behaviors and the seasonally changing local environment affect malaria transmission. Field studies in Mali will be conducted at 10 study sites located in 3 major eco-climatic zones that span the sub-Saharan Sahel region. Study areas are already well characterized with respect to vector populations, seasonal Plasmodium falciparum transmission, and malaria epidemiology. This project, which builds on extensive preliminary studies in Mali and Israel, includes three specific aims: 1) Characterize how malaria vector species select from different types, configurations, and qualities of potential outdoor resting microhabitats and sugar-feeding centers, and behaviorally adapt to seasonal changes in their local environment~ 2) Determine how local environmental resources critical for malaria vector outdoor resting and sugar feeding affect malaria vectorial capacity and spatial aggregation patterns of malaria parasite transmission~ and 3) Develop and evaluate new approaches and field-based criteria to identify concentrations of adult anopheline mosquitoes in the outdoor environment. Innovative aspects include: First, new field-based approaches will be used to investigate two important but highly neglected mosquito behaviors, Second, this is the first study to examine how mosquitoes adapt their outdoor-resting and sugar-feeding behaviors in response to seasonal changes in their local environment. Third, this project will explain how outdoor-resting and sugar-feeding behaviors impact vector survival and malaria parasite transmission dynamics. Fourth, new predictive models for identifying concentrations of malaria vectors will be rigorously field-tested and refined to improve their accuracy and potential utility beyond Mali. By providing a strong scientific basis for development of new approaches and tools for anopheline mosquito vector control in outdoor environments, this project has strong potential for helping Mali and other countries improve malaria control and advance toward the ultimate goal of elimination.

Public Health Relevance

This project focuses on the outdoor resting and sugar-feeding behaviors of vector species of Anopheles mosquitoes at malaria endemic sites in Mali, West Africa. It addresses the need to better understand how adult mosquitoes adapt to seasonally changing environmental conditions, how limitations in critical environmental resources including vegetated habitats and flowering plants impact malaria transmission, and the challenge of developing new geographical approaches and tools to locate outdoor concentrations of adult mosquitoes. This research will contribute to more effective outdoor vector control to reduce malaria transmission in Africa.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
1R01AI100968-01A1
Application #
8515715
Study Section
Special Emphasis Panel (ZRG1-IDM-M (02))
Program Officer
Costero, Adriana
Project Start
2013-03-01
Project End
2018-02-28
Budget Start
2013-03-01
Budget End
2014-02-28
Support Year
1
Fiscal Year
2013
Total Cost
$564,304
Indirect Cost
$87,969
Name
University of Miami School of Medicine
Department
Public Health & Prev Medicine
Type
Schools of Medicine
DUNS #
052780918
City
Coral Gables
State
FL
Country
United States
Zip Code
33146
Coulibaly, C A; Sissoko, I; Traore, B et al. (2016) Diversity of Sand Flies (Diptera: Psychodidae: Phlebotominae) in Two Different Eco-Climatic and Endemic Zones of Cutaneous Leishmaniasis in Mali, West Africa. J Med Entomol 53:923-927
Müller, Günter C; Tsabari, Onie; Traore, Mohamed M et al. (2016) First record of Aedes albopictus in inland Africa along the River Niger in Bamako and Mopti, Mali. Acta Trop 162:245-7
Dewald, Julius R; Fuller, Douglas O; Müller, Günter C et al. (2016) A novel method for mapping village-scale outdoor resting microhabitats of the primary African malaria vector, Anopheles gambiae. Malar J 15:489
Qualls, Whitney A; Müller, Gunter C; Khallaayoune, Khalid et al. (2015) Control of sand flies with attractive toxic sugar baits (ATSB) and potential impact on non-target organisms in Morocco. Parasit Vectors 8:87
Zhu, Lin; Marshall, John M; Qualls, Whitney A et al. (2015) Modelling optimum use of attractive toxic sugar bait stations for effective malaria vector control in Africa. Malar J 14:492
Revay, Edita E; Schlein, Yosef; Tsabari, Onie et al. (2015) Formulation of attractive toxic sugar bait (ATSB) with safe EPA-exempt substance significantly diminishes the Anopheles sergentii population in a desert oasis. Acta Trop 150:29-34
Samson, Dayana M; Archer, Reginald S; Alimi, Temitope O et al. (2015) New baseline environmental assessment of mosquito ecology in northern Haiti during increased urbanization. J Vector Ecol 40:46-58
Qualls, Whitney A; Müller, Günter C; Traore, Sekou F et al. (2015) Indoor use of attractive toxic sugar bait (ATSB) to effectively control malaria vectors in Mali, West Africa. Malar J 14:301
Junnila, Amy; Revay, Edita E; Müller, Gunter C et al. (2015) Efficacy of attractive toxic sugar baits (ATSB) against Aedes albopictus with garlic oil encapsulated in beta-cyclodextrin as the active ingredient. Acta Trop 152:195-200
Zhu, Lin; Qualls, Whitney A; Marshall, John M et al. (2015) A spatial individual-based model predicting a great impact of copious sugar sources and resting sites on survival of Anopheles gambiae and malaria parasite transmission. Malar J 14:59

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