We have identified more than 40,000 single nucleotide polymorphisms (SNPs) in 16 samples of Aedes aegypti from around the world, as well as a sample of its closest relative, Ae. mascarensis. This was done using a technique that is efficient in identifying SNPs (RAD-tags), but this methodology is not applicable for routine genotyping. We propose to develop a SNP chip based on these identified SNPs that can be applied routinely for reliable genotyping in a time- and cost-effective manner. Our own interests are on the population genetics and ancestry level and we will focus on a chip designed specifically for our studies. A by-product of the work will be more extensive data upon which other groups could design a chip for genome wide association studies;all data will be publically available. The first part of the project is development of the population chip. This will proceed i three steps: (1) From the >40,000 available SNPs, identify ~5,000 that have the most variation across the species'distribution and reliably genotype on SNP chips. (2) From these 5,000, use theoretical analyses to identify ~750 that capture the population/ancestry information of the complete data set. (2) Perform crosses to confirm the Mendelian (single-copy, nuclear) nature of these 750, with the expectation that at least 500 will be Mendelian and form the basis of the population SNP chip. The second part of the project is application of this chip. Four projects are proposed: (1) Perform a global population genetics study to determine the genetic diversity, patterns, and relatedness of populations from around the world;collections are already in hand for this. (2) Study five ecologically and geographically diverse populations through time to determine the genetic stability of Ae. aegypti populations as well as effective population sizes. (3) Genotype common lab strains to determine where they fit into the genetic diversity of the species and how homogeneous or heterogeneous they are among isolates from different laboratories. (4) Genotype populations in West Africa that have recently colonized urban centers and compare them to nearby sylvan populations that are the likely source of the "domestication" event;independent replicate cases will allow us to identify parallel genetic changes that would give insight into the genetic nature of the domestication process.

Public Health Relevance

Aedes aegypti, the yellow fever mosquito, is more feared today as the major vector of dengue fever, a viral disease that threatens fully 40% of the global population. We propose to use the most up-to-date technologies to genetically characterize this mosquito that exhibits extremely high diversity in terms of ecology (breeding in forests, towns, and urban centers) and behavior (e.g., biting animals in some localities, humans in others).

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI101112-02
Application #
8479317
Study Section
Vector Biology Study Section (VB)
Program Officer
Costero, Adriana
Project Start
2012-06-15
Project End
2017-05-31
Budget Start
2013-06-01
Budget End
2014-05-31
Support Year
2
Fiscal Year
2013
Total Cost
$391,177
Indirect Cost
$156,177
Name
Yale University
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
043207562
City
New Haven
State
CT
Country
United States
Zip Code
06520
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Gloria-Soria, Andrea; Brown, Julia E; Kramer, Vicki et al. (2014) Origin of the dengue fever mosquito, Aedes aegypti, in California. PLoS Negl Trop Dis 8:e3029
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Monteiro, Fernando A; Schama, Renata; Shama, Renata et al. (2014) Genetic diversity of Brazilian Aedes aegypti: patterns following an eradication program. PLoS Negl Trop Dis 8:e3167
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