We have identified more than 40,000 single nucleotide polymorphisms (SNPs) in 16 samples of Aedes aegypti from around the world, as well as a sample of its closest relative, Ae. mascarensis. This was done using a technique that is efficient in identifying SNPs (RAD-tags), but this methodology is not applicable for routine genotyping. We propose to develop a SNP chip based on these identified SNPs that can be applied routinely for reliable genotyping in a time- and cost-effective manner. Our own interests are on the population genetics and ancestry level and we will focus on a chip designed specifically for our studies. A by-product of the work will be more extensive data upon which other groups could design a chip for genome wide association studies;all data will be publically available. The first part of the project is development of the population chip. This will proceed i three steps: (1) From the >40,000 available SNPs, identify ~5,000 that have the most variation across the species'distribution and reliably genotype on SNP chips. (2) From these 5,000, use theoretical analyses to identify ~750 that capture the population/ancestry information of the complete data set. (2) Perform crosses to confirm the Mendelian (single-copy, nuclear) nature of these 750, with the expectation that at least 500 will be Mendelian and form the basis of the population SNP chip. The second part of the project is application of this chip. Four projects are proposed: (1) Perform a global population genetics study to determine the genetic diversity, patterns, and relatedness of populations from around the world;collections are already in hand for this. (2) Study five ecologically and geographically diverse populations through time to determine the genetic stability of Ae. aegypti populations as well as effective population sizes. (3) Genotype common lab strains to determine where they fit into the genetic diversity of the species and how homogeneous or heterogeneous they are among isolates from different laboratories. (4) Genotype populations in West Africa that have recently colonized urban centers and compare them to nearby sylvan populations that are the likely source of the """"""""domestication"""""""" event;independent replicate cases will allow us to identify parallel genetic changes that would give insight into the genetic nature of the domestication process.

Public Health Relevance

Aedes aegypti, the yellow fever mosquito, is more feared today as the major vector of dengue fever, a viral disease that threatens fully 40% of the global population. We propose to use the most up-to-date technologies to genetically characterize this mosquito that exhibits extremely high diversity in terms of ecology (breeding in forests, towns, and urban centers) and behavior (e.g., biting animals in some localities, humans in others).

National Institute of Health (NIH)
National Institute of Allergy and Infectious Diseases (NIAID)
Research Project (R01)
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Vector Biology Study Section (VB)
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Costero, Adriana
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Yale University
Schools of Arts and Sciences
New Haven
United States
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Powell, Jeffrey R (2018) Mosquito-Borne Human Viral Diseases: Why Aedes aegypti? Am J Trop Med Hyg 98:1563-1565
Kotsakiozi, Panayiota; Gloria-Soria, Andrea; Schaffner, Francis et al. (2018) Aedes aegypti in the Black Sea: recent introduction or ancient remnant? Parasit Vectors 11:396
Kotsakiozi, Panayiota; Evans, Benjamin R; Gloria-Soria, Andrea et al. (2018) Population structure of a vector of human diseases: Aedes aegypti in its ancestral range, Africa. Ecol Evol 8:7835-7848
Gloria-Soria, Andrea; Chiodo, Tommaso G; Powell, Jeffrey R (2018) Lack of Evidence for Natural Wolbachia Infections in Aedes aegypti (Diptera: Culicidae). J Med Entomol 55:1354-1356
Gloria-Soria, Andrea; Lima, Andrew; Lovin, Diane D et al. (2018) Origin of a High-Latitude Population of Aedes aegypti in Washington, DC. Am J Trop Med Hyg 98:445-452
Saarman, Norah P; Gloria-Soria, Andrea; Anderson, Eric C et al. (2017) Effective population sizes of a major vector of human diseases, Aedes aegypti. Evol Appl 10:1031-1039
Kotsakiozi, Panayiota; Gloria-Soria, Andrea; Caccone, Adalgisa et al. (2017) Tracking the return of Aedes aegypti to Brazil, the major vector of the dengue, chikungunya and Zika viruses. PLoS Negl Trop Dis 11:e0005653
Pless, Evlyn; Gloria-Soria, Andrea; Evans, Benjamin R et al. (2017) Multiple introductions of the dengue vector, Aedes aegypti, into California. PLoS Negl Trop Dis 11:e0005718
IbaƱez-Justicia, A; Gloria-Soria, A; den Hartog, W et al. (2017) The first detected airline introductions of yellow fever mosquitoes (Aedes aegypti) to Europe, at Schiphol International airport, the Netherlands. Parasit Vectors 10:603
Gloria-Soria, A; Kellner, D A; Brown, J E et al. (2016) Temporal genetic stability of Stegomyia aegypti (= Aedes aegypti) populations. Med Vet Entomol 30:235-40

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