The global malaria burden remains high despite recent advances in malaria control efforts. The goal of this project is to develop data-driven tool to enable malaria control programs to target the malaria transmission reservoir. We will use samples from the Democratic Republic of the Congo, one of countries with the highest malaria burden in the world. Dried blood spots will be obtained a large cross-sectional study, the 2014 Demographic and Health Survey (DHS), and from a new prospective study of 1000 individuals in 4 sites with varying levels of endemicity. Gametocytemia will be measured by reverse transcriptase PCR.
Our first aim i s to map the gametocyte reservoir and determine how individual, community-level, and ecological factors determine an individual's risk for gametocyte carriage.
Our second aim i s to identify barriers and corridors of gene flow. The structure of the P. falciparum at each cluster in the DHS will be assessed using neutral microsatellites. The genetic distances between parasite subpopulations will be calculated using population genetic metrics (Rst, Gst, Nm) and used to infer corridors of, and barriers to, gene flow.
Our third aim i s to use the results from aims 1 and 2 to parameterize and improve an existing mathematical spatial model for malaria transmission. This latter model will be developed into a user-friendly freely available PC-based tool to allow the impact of targeted control to be explored in DRC and the surrounding region. This project represents a unique multidisciplinary collaboration between molecular epidemiologists, medical geographers and mathematical modelers. By incorporating results from molecular and spatial epidemiological studies into mathematical models, we hope to develop a useful and practical tool for malaria control.
Malaria represents a huge global health problem, especially in the Democratic Republic of the Congo (DRC) where 1/3 of adults may be infected. Malaria control efforts need to target the transmission reservoir, people bearing the gametocyte lifecycle stage. The aim of this proposal is to develop maps and tools to enable malaria control programs to predict how malaria spreads over space and how to target hotspots of transmission
|Miller, Robin H; Hathaway, Nicholas J; Kharabora, Oksana et al. (2017) A deep sequencing approach to estimate Plasmodium falciparum complexity of infection (COI) and explore apical membrane antigen 1 diversity. Malar J 16:490|
|Parr, Jonathan B; Verity, Robert; Doctor, Stephanie M et al. (2017) Pfhrp2-Deleted Plasmodium falciparum Parasites in the Democratic Republic of the Congo: A National Cross-sectional Survey. J Infect Dis 216:36-44|
|Juliano, Jonathan J; Parobek, Christian M; Brazeau, Nicholas F et al. (2016) Pooled Amplicon Deep Sequencing of Candidate Plasmodium falciparum Transmission-Blocking Vaccine Antigens. Am J Trop Med Hyg 94:143-6|
|Doctor, Stephanie M; Liu, Yunhao; Whitesell, Amy et al. (2016) Malaria surveillance in the Democratic Republic of the Congo: comparison of microscopy, PCR, and rapid diagnostic test. Diagn Microbiol Infect Dis 85:16-8|
|Liu, Yunhao; Griffin, Jennifer B; Muehlenbachs, Atis et al. (2016) Diagnosis of placental malaria in poorly fixed and processed placental tissue. Malar J 15:272|
|Doctor, Stephanie M; Liu, Yunhao; Anderson, Olivia G et al. (2016) Low prevalence of Plasmodium malariae and Plasmodium ovale mono-infections among children in the Democratic Republic of the Congo: a population-based, cross-sectional study. Malar J 15:350|
|Carrel, Margaret; Janko, Mark; Mwandagalirwa, Melchior Kashamuka et al. (2016) Changing spatial patterns and increasing rurality of HIV prevalence in the Democratic Republic of the Congo between 2007 and 2013. Health Place 39:79-85|
|Brazeau, Nicholas F; Tabala, Martine; Kiketa, Landry et al. (2016) Exclusive Breastfeeding and Clinical Malaria Risk in 6-Month-Old Infants: A Cross-Sectional Study from Kinshasa, Democratic Republic of the Congo. Am J Trop Med Hyg 95:827-830|
|Carrel, Margaret; Patel, Jaymin; Taylor, Steve M et al. (2015) The geography of malaria genetics in the Democratic Republic of Congo: A complex and fragmented landscape. Soc Sci Med 133:233-41|
|Lin, Jessica T; Saunders, David L; Meshnick, Steven R (2014) The role of submicroscopic parasitemia in malaria transmission: what is the evidence? Trends Parasitol 30:183-90|
Showing the most recent 10 out of 12 publications