Quantifying Environmental Variables Affecting Airborne Influenza Transmission In most temperate climates, influenza prevails in cold, dry winter months. However, in some temperate and tropical regions, influenza epidemicity is correlated with extremes of precipitation, not dryness, and can circulate at low levels essentially year-round or appear in uni- or bi-modal annual outbreaks. How environmental variables affect influenza circulation in the human population remains poorly understood, in part because the science behind airborne respiratory virus transmission crosses disciplinary boundaries between the biomedical and physical sciences, encompassing fields as diverse as virology, physiology, epidemiology, fluid mechanics, aerosol science, and climatology. Here we seek to understand how individual environmental variables - such as temperature, humidity, and airflow - cumulatively affect the transmission probability of influenza viruses in a representative mammalian experimental system. The theoretical framework behind these studies is a novel quantitative model, based upon data gathered in experimental guinea pigs, which attempts to characterize the impact of the environment on influenza virus transmission between infected and susceptible hosts. This project bridges the gap between virology and engineering in bringing together three co- investigators with relevant and complementary skill sets: Dr. Nicole Bouvier, a physician-scientist with extensive experience in the transmission of influenza viruses among guinea pigs; Dr. William Ristenpart, an engineer with expertise in the application of high-speed imaging technologies to investigations of complex fluid dynamics; and Dr. Anthony Wexler, an authority on aerosol transport who has developed novel techniques for high-resolution imaging of aerosol deposition in the rodent respiratory tract. Our preliminary theoretical modeling has generated innovative interpretations of the experimental data, yielding three testable hypotheses, which form the basis of this proposal: (1) Influenza virus transmission probability will decrease with increased airflow speed, (2) transmission probability will decrease with the degree of turbulence, and (3) transmission probability will increase with the time integral of the viral concentration within the inoculated animal. Rigorously controlled laboratory studies, designed to isolate a single variable for analysis while others are held constant, will provide a quantitative framework for understanding the cumulative effects of temperature, humidity, airflow velocity, turbulence, and position on the transmission of human influenza viruses in a relevant animal model. Quantifying these environmental variables, individually and cumulatively, will enable their extrapolation to larger environments and time scales, with the potential to transform our understanding of the epidemiology of seasonal and pandemic influenza.

Public Health Relevance

Quantifying Environmental Variables Affecting Airborne Influenza Transmission This project addresses questions of critical importance in understanding the circulation of influenza viruses in the human population. Our main scientific goal is to perform experiments that explain how environmental variables - such as temperature, humidity, and airflow - affect the ability of influenza viruses to spread among experimental guinea pigs; these results can then be analyzed mathematically so that we can better appreciate the cumulative effect of each individual variable on influenza transmission as a whole. Improved understanding of these variables will aid in designing public health interventions designed to interrupt the spread of influenza viruses among people, which has an enormous economic and health impact on populations worldwide.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI110703-05
Application #
9591336
Study Section
Virology - B Study Section (VIRB)
Program Officer
Hauguel, Teresa M
Project Start
2014-11-05
Project End
2019-10-31
Budget Start
2018-11-01
Budget End
2019-10-31
Support Year
5
Fiscal Year
2019
Total Cost
Indirect Cost
Name
Icahn School of Medicine at Mount Sinai
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
078861598
City
New York
State
NY
Country
United States
Zip Code
10029
Bouvier, Nicole M (2016) Cystic fibrosis and the war for iron at the host-pathogen battlefront. Proc Natl Acad Sci U S A 113:1480-2
Bouvier, Nicole M (2015) Animal models for influenza virus transmission studies: a historical perspective. Curr Opin Virol 13:101-8