Biogenesis of Membrane Lipids in Animal Cells

Raetz, Christian R.

Abstract

The present study has as its objective to understand the regulation, biosynthesis, and function of membrane phospholipids in mammalian systems. Toward this end we are employing a combination of genetics and biochemistry by utilizing Chinese hamster ovary (CHO) cells. These have the advantage that: 1) they can be cloned from single cells; and 2) biochemically-defined mutants can be isolated in which the membrane composition is altered.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Arthritis, Diabetes, Digestive and Kidney Diseases (NIADDK)
• Type: Research Project (R01)
• Project #: 5R01AM021722-08
• Application #: 3151404
• Study Section: Biochemistry Study Section (BIO)
• Project Start: 1978-07-01
• Project End: 1986-06-30
• Budget Start: 1985-07-01
• Budget End: 1986-06-30
• Support Year: 8
• Fiscal Year: 1985

Institution

• Name: University of Wisconsin Madison
• Type: Earth Sciences/Resources
• DUNS #: 161202122
• City: Madison
• State: WI
• Country: United States
• Zip Code: 53715

Publications

Golenbock, D T; Leggett, J E; Rasmussen, P et al. (1988) Lipid X protects mice against fatal Escherichia coli infection. Infect Immun 56:779-84

Ray, B L; Raetz, C R (1987) The biosynthesis of gram-negative endotoxin. A novel kinase in Escherichia coli membranes that incorporates the 4'-phosphate of lipid A. J Biol Chem 262:1122-8

Golenbock, D T; Will, J A; Raetz, C R et al. (1987) Lipid X ameliorates pulmonary hypertension and protects sheep from death due to endotoxin. Infect Immun 55:2471-6

Raetz, C R (1986) Molecular genetics of membrane phospholipid synthesis. Annu Rev Genet 20:253-95

Gum Jr, J R; Raetz, C R (1985) Biochemical and immunological characterization of mutant L-M cells with altered levels of dibutyryl cyclic AMP-inducible alkaline phosphatase. Mol Cell Biol 5:1184-7

Proctor, R A; Textor, J A (1985) Activation and inhibition of Limulus amebocyte lysate coagulation by chemically defined substructures of lipid A. Infect Immun 49:286-90

Nishijima, M; Amano, F; Akamatsu, Y et al. (1985) Macrophage activation by monosaccharide precursors of Escherichia coli lipid A. Proc Natl Acad Sci U S A 82:282-6