The goal of this proposal is to study the dynamics of Ca++ trafficking through cellular organelles and its relationship to the regulation of exocytosis using a combination of high time resolution patch clamp, capacitance measurements, amperometry, photometry and imaging. This will be done in single cells and adrenal slice preparations.
The specific aims are: 1. Dynamics of Ca++ movement into and out of organelles. Specific hypotheses addressed are that organelles play significant roles in Ca++ homeostasis and that chromaffin secretory granules are Ca++ releasing and sequestering organelles. 2. Signaling pathway for regulated exocytosis in epithelia. The hypothesis that a cAMP-dependent pathway controls exocytosis will be tested. 3. Physiology of chromaffin cells. The hypothesis that chromaffin secretion is regulated by paracrine and modulatory actions will be tested.

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Research Project (R01)
Project #
5R01AR017803-24
Application #
2683254
Study Section
Physiology Study Section (PHY)
Project Start
1977-09-01
Project End
2002-03-31
Budget Start
1998-04-01
Budget End
1999-03-31
Support Year
24
Fiscal Year
1998
Total Cost
Indirect Cost
Name
University of Washington
Department
Physiology
Type
Schools of Medicine
DUNS #
135646524
City
Seattle
State
WA
Country
United States
Zip Code
98195
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Cho, Jung-Hwa; Chen, Liangyi; Kim, Mean-Hwan et al. (2010) Characteristics and functions of {alpha}-amino-3-hydroxy-5-methyl-4-isoxazolepropionate receptors expressed in mouse pancreatic {alpha}-cells. Endocrinology 151:1541-50
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Horowitz, Lisa F; Hirdes, Wiebke; Suh, Byung-Chang et al. (2005) Phospholipase C in living cells: activation, inhibition, Ca2+ requirement, and regulation of M current. J Gen Physiol 126:243-62

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