The focus of this project is to examine physiological mechanisms that regulate human skeletal muscle growth. Muscle wasting is a common phenomenon in many conditions, such as cancer, AIDS, trauma, sepsis, kidney failure, and is also particularly prevalent in the elderly. The loss of muscle mass leads to overall weakness, immobility and physical dependence, and can be responsible for an impaired response to stress, which is associated with higher mortality rates during infection, surgery, and trauma. Nutrition and resistance exercise are two major protein anabolic stimuli for skeletal muscle. Our general hypothesis is that the magnitude of the protein anabolic response will be determined by the reciprocal interactions between these two stimuli. In particular, we propose that skeletal muscle protein accretion is dependent upon both the timing of the nutritional stimulus in relation to the exercise bout and the amount of energy provided by the nutritional stimulus. We further hypothesize that the muscle protein anabolic response following a nutrient stimulus or muscular contraction will be more pronounced in healthy young men than in young women.
Specific aims : (1) To determine the magnitude of the acute response of skeletal muscle protein synthesis and net protein anabolism to a nutritional stimulus given before or after a bout of resistance exercise. (2) To determine the magnitude of the acute response of skeletal muscle protein synthesis and net protein anabolism to a nutritional stimulus providing an amount of energy that either matches or exceeds the exercise-induced energy expenditure. (3) To determine the differences between women and men in the response of skeletal muscle protein synthesis, efficiency of amino acid utilization for protein synthesis, leucine oxidation, and net protein anabolism to resistance exercise alone and in combination with a nutritional stimulus delivered before or after a bout of resistance exercise. Significance. These studies will provide insight into physiological mechanisms that regulate human muscle protein balance, and will be utilized as a basis from which to develop scientificallybased interventions for improving muscle protein balance in conditions such as aging, trauma, cancer, and AIDS.

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Research Project (R01)
Project #
5R01AR049877-04
Application #
7276663
Study Section
Nutrition Study Section (NTN)
Program Officer
Boyce, Amanda T
Project Start
2004-09-01
Project End
2008-09-14
Budget Start
2007-09-01
Budget End
2008-09-14
Support Year
4
Fiscal Year
2007
Total Cost
$293,151
Indirect Cost
Name
University of Texas Medical Br Galveston
Department
Other Health Professions
Type
Schools of Allied Health Profes
DUNS #
800771149
City
Galveston
State
TX
Country
United States
Zip Code
77555
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Graber, Ted G; Borack, Michael S; Reidy, Paul T et al. (2017) Essential amino acid ingestion alters expression of genes associated with amino acid sensing, transport, and mTORC1 regulation in human skeletal muscle. Nutr Metab (Lond) 14:35
Reidy, Paul T; Fry, Christopher S; Igbinigie, Sherry et al. (2017) Protein Supplementation Does Not Affect Myogenic Adaptations to Resistance Training. Med Sci Sports Exerc 49:1197-1208
Reidy, Paul T; Borack, Michael S; Markofski, Melissa M et al. (2017) Post-absorptive muscle protein turnover affects resistance training hypertrophy. Eur J Appl Physiol 117:853-866
Porter, Craig; Herndon, David N; Børsheim, Elisabet et al. (2016) Long-Term Skeletal Muscle Mitochondrial Dysfunction is Associated with Hypermetabolism in Severely Burned Children. J Burn Care Res 37:53-63
Ogunbileje, John O; Porter, Craig; Herndon, David N et al. (2016) Hypermetabolism and hypercatabolism of skeletal muscle accompany mitochondrial stress following severe burn trauma. Am J Physiol Endocrinol Metab 311:E436-48
Reidy, Paul T; Rasmussen, Blake B (2016) Role of Ingested Amino Acids and Protein in the Promotion of Resistance Exercise-Induced Muscle Protein Anabolism. J Nutr 146:155-83
Porter, Craig; Herndon, David N; Chondronikola, Maria et al. (2016) Human and Mouse Brown Adipose Tissue Mitochondria Have Comparable UCP1 Function. Cell Metab 24:246-55
Saraf, Manish Kumar; Herndon, David N; Porter, Craig et al. (2016) Morphological Changes in Subcutaneous White Adipose Tissue After Severe Burn Injury. J Burn Care Res 37:e96-103
Coble, Joel; Schilder, Rudolf J; Berg, Arthur et al. (2015) Influence of ageing and essential amino acids on quantitative patterns of troponin T alternative splicing in human skeletal muscle. Appl Physiol Nutr Metab 40:788-796

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