FSHD affects over 25,000 individuals in the United States. It is the third most common muscular dystrophy by incidence but may be the most common by prevalence (Orphanet, 2008). The DNA lesion associated with this disease is a contraction within a series of 3.3 kb repeats (D4Z4 repeats) near the telomere of 4q. The contraction modifies the chromatin configuration of 4q35.2 which results in misexpression of a gene encoded within each D4Z4 repeat, DUX4. We have shown that DUX4 is cytotoxic when expressed at high levels in various cellular model systems, and interferes with myogenic gene expression when expressed at low levels in satellite cells and myoblasts. However mechanistically, DUX4 is not well understood, we do not have a clear picture of which cell types in muscle express DUX4 and what the consequence of that expression is, and a pathological mechanism still eludes the field. The research proposed in this application addresses these issues by (1) probing the activity of cofactors of DUX4 in transcription, (2) investigating the ste cell compartment of skeletal muscle of FSHD patients, and (3) modeling DUX4 expression and D4Z4 regulation in mouse. This research will address key outstanding questions in FSHD, will advance a mechanistic understanding of DUX4 in FSHD at the molecular, cellular, and tissue levels, and may lead to both an improved animal model and new therapeutic directions.

Public Health Relevance

Facioscapulohumeral muscular dystrophy (FSHD) a genetically dominant progressive muscular dystrophy associated with derepression of the DUX4 gene. DUX4 causes myoblasts to become sensitive to oxidative stress, and interferes with myogenic pathways. This application focuses on understanding how this protein works and developing models to study its effects on human cells and in animal models. This work also has the potential to lead to therapies for FSHD based in inhibiting DUX4 activity.

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Research Project (R01)
Project #
2R01AR055685-06A1
Application #
8968701
Study Section
Skeletal Muscle Biology and Exercise Physiology Study Section (SMEP)
Program Officer
Cheever, Thomas
Project Start
2007-12-01
Project End
2020-06-30
Budget Start
2015-07-15
Budget End
2016-06-30
Support Year
6
Fiscal Year
2015
Total Cost
Indirect Cost
Name
University of Minnesota Twin Cities
Department
Pediatrics
Type
Schools of Medicine
DUNS #
555917996
City
Minneapolis
State
MN
Country
United States
Zip Code
55455
Bosnakovski, Darko; Toso, Erik A; Hartweck, Lynn M et al. (2017) The DUX4 homeodomains mediate inhibition of myogenesis and are functionally exchangeable with the Pax7 homeodomain. J Cell Sci 130:3685-3697
Bosnakovski, Darko; Gearhart, Micah D; Toso, Erik A et al. (2017) p53-independent DUX4 pathology in cell and animal models of facioscapulohumeral muscular dystrophy. Dis Model Mech 10:1211-1216
Arpke, Robert W; Kyba, Michael (2016) Flow Cytometry and Transplantation-Based Quantitative Assays for Satellite Cell Self-Renewal and Differentiation. Methods Mol Biol 1460:163-79
Choi, Si Ho; Gearhart, Micah D; Cui, Ziyou et al. (2016) DUX4 recruits p300/CBP through its C-terminus and induces global H3K27 acetylation changes. Nucleic Acids Res 44:5161-73
Choi, Si Ho; Bosnakovski, Darko; Strasser, Jessica M et al. (2016) Transcriptional Inhibitors Identified in a 160,000-Compound Small-Molecule DUX4 Viability Screen. J Biomol Screen 21:680-8
Zhang, Yu; Lee, John K; Toso, Erik A et al. (2016) DNA-binding sequence specificity of DUX4. Skelet Muscle 6:8
Le, Gengyun; Lowe, Dawn A; Kyba, Michael (2016) Freeze Injury of the Tibialis Anterior Muscle. Methods Mol Biol 1460:33-41
Dandapat, Abhijit; Perrin, Benjamin J; Cabelka, Christine et al. (2016) High Frequency Hearing Loss and Hyperactivity in DUX4 Transgenic Mice. PLoS One 11:e0151467
Kyba, Michael (2016) Mesoderm, Cooked Up Fast and Served to Order. Cell Stem Cell 19:146-148
Filareto, Antonio; Rinaldi, Fabrizio; Arpke, Robert W et al. (2015) Pax3-induced expansion enables the genetic correction of dystrophic satellite cells. Skelet Muscle 5:36

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