The mammalian somatosensory system detects a wide variety of mechanical stimuli, such as texture, shape, vibration or pressure. This variety of stimuli is matched by a diverse array of mechanosensitive somatosensory neurons. Non-neuronal cells in the skin, such as keratinocytes, may also coordinate with sensory neurons to transduce force. The goal of this proposal is to identify molecular and cellular mechanisms underlying somatosensory mechanotransduction. We hypothesize that both primary afferent somatosensory neurons and keratinocytes mediate mechanosensitive responses in the skin. We will use a variety of techniques including live-cell Ca2+ imaging, electrophysiology and pharmacology to characterize transduction channels in sensory neurons and keratinocytes. We will then probe the role of candidate channels in vivo. The proposed experiments will answer fundamental questions about somatosensory mechanotransduction, including: 1) What are the molecular identities of the ion channels that transduce touch in sensory neurons and keratinocytes? and 2) How does touch-evoked signaling in keratinocytes alter primary afferent neuron function?

Public Health Relevance

Though unpleasant, pain warns us against harmful stimuli in the environment and evokes protective reflexes. But pain can also be a chronic, debilitating affliction that no longer serves a protective purpose. Chronic pain not only occurs after trauma-induced inflammation and tissue injury, but also results from many diseases;pain is the major complaint of patients suffering from cancer, AIDS, and diabetes. Understanding the mechanisms that evoke acute and chronic pain may lead to the development of much needed, novel drugs and therapies to alleviate pain.

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Research Project (R01)
Project #
5R01AR059385-02
Application #
8296042
Study Section
Neurotransporters, Receptors, and Calcium Signaling Study Section (NTRC)
Program Officer
Tseng, Hung H
Project Start
2011-07-01
Project End
2016-04-30
Budget Start
2012-05-01
Budget End
2013-04-30
Support Year
2
Fiscal Year
2012
Total Cost
$338,401
Indirect Cost
$113,401
Name
University of California Berkeley
Department
Biochemistry
Type
Schools of Arts and Sciences
DUNS #
124726725
City
Berkeley
State
CA
Country
United States
Zip Code
94704
Bautista, Diana M; Wilson, Sarah R; Hoon, Mark A (2014) Why we scratch an itch: the molecules, cells and circuits of itch. Nat Neurosci 17:175-82
Wilson, Sarah R; The, Lydia; Batia, Lyn M et al. (2013) The epithelial cell-derived atopic dermatitis cytokine TSLP activates neurons to induce itch. Cell 155:285-95
Tsunozaki, Makoto; Lennertz, Richard C; Vilceanu, Daniel et al. (2013) A 'toothache tree' alkylamide inhibits Aýý mechanonociceptors to alleviate mechanical pain. J Physiol 591:3325-40
Bautista, Diana M; Pellegrino, Maurizio; Tsunozaki, Makoto (2013) TRPA1: A gatekeeper for inflammation. Annu Rev Physiol 75:181-200
Wilson, Sarah R; Nelson, Aislyn M; Batia, Lyn et al. (2013) The ion channel TRPA1 is required for chronic itch. J Neurosci 33:9283-94
Almeida, M Camila; Hew-Butler, Tamara; Soriano, Renato N et al. (2012) Pharmacological blockade of the cold receptor TRPM8 attenuates autonomic and behavioral cold defenses and decreases deep body temperature. J Neurosci 32:2086-99
Bautista, Diana M; Lumpkin, Ellen A (2011) Perspectives on: information and coding in mammalian sensory physiology: probing mammalian touch transduction. J Gen Physiol 138:291-301