The objective of this proposal is to develop reconstituted skin that can form new hairs from dissociated stem cells. Progress has been made in producing hair follicles using dissociated multi-potential dermal and epidermal stem cells. However, couple gaps need to be filled before significant progress in clinical applications can be made. Recently, we have improved significantly the previous assays by Lichti and Stenn into a simpler hair forming assay with clinically proper appearance and potential for a high throughput assay. Our general hypothesis is that multi-potent skin stem cells can be generated by molecular reprogramming, and that arrangement and patterning of hair primordia can be regulated by environmental conditions. With the newly developed "planar hair forming procedure", we are able to apply our expertise in feather pattern formation to regulate the number and size of hair primordia. We will also apply our recent experience on macro-environment regulation of hair stem cells to improve the efficiency of hair formation. Recent success of molecular re-programmig in other organs has inspired excitement. This simple assay will allow us to do high throughput analyses to identify molecules or combinations of molecules important to reprogram adult skin cells into hair forming dermal and epidermal cells. The development of this technology will bring new hope to those who suffer from severe wounds and alopecia.

Public Health Relevance

Currently, over 100,000 patients are treated for burn injury in the United States per year, and burns can cause lasting cosmetic and functional defects to visible areas on the skin. Here we propose to develop a novel planar hair forming protocol which integrates progress from our parent grant and others hair research and may bring new hope to those who suffer from severe wounds, and alopecia.

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Research Project (R01)
Project #
5R01AR060306-03
Application #
8314050
Study Section
Musculoskeletal Tissue Engineering Study Section (MTE)
Program Officer
Baker, Carl
Project Start
2010-09-21
Project End
2015-08-31
Budget Start
2012-09-01
Budget End
2013-08-31
Support Year
3
Fiscal Year
2012
Total Cost
$349,920
Indirect Cost
$133,920
Name
University of Southern California
Department
Pathology
Type
Schools of Medicine
DUNS #
072933393
City
Los Angeles
State
CA
Country
United States
Zip Code
90089
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Chen, Chih-Chiang; Murray, Philip J; Jiang, Ting Xin et al. (2014) Regenerative hair waves in aging mice and extra-follicular modulators follistatin, dkk1, and sfrp4. J Invest Dermatol 134:2086-96
Wu, Ping; Alibardi, Lorenzo; Chuong, Cheng-Ming (2014) Regeneration of reptilian scales after wounding: neogenesis, regional difference, and molecular modules. Regeneration (Oxf) 1:15-26
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Driskell, Ryan R; Jahoda, Colin A B; Chuong, Cheng-Ming et al. (2014) Defining dermal adipose tissue. Exp Dermatol 23:629-31
Kandyba, Eve; Leung, Yvonne; Chen, Yi-Bu et al. (2013) Competitive balance of intrabulge BMP/Wnt signaling reveals a robust gene network ruling stem cell homeostasis and cyclic activation. Proc Natl Acad Sci U S A 110:1351-6
Chuong, Cheng-Ming; Yeh, Chao-Yuan; Jiang, Ting-Xin et al. (2013) Module-based complexity formation: periodic patterning in feathers and hairs. Wiley Interdiscip Rev Dev Biol 2:97-112
Li, Ang; Chen, Meng; Jiang, Ting-Xin et al. (2013) Shaping organs by a wingless-int/Notch/nonmuscle myosin module which orients feather bud elongation. Proc Natl Acad Sci U S A 110:E1452-61

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