System lupus erythematosus (SLE) is an autoimmune disorder of complex etiology characterized by diverse immune cell dysfunction. Monogenic SLE models are uniquely useful for molecular dissection of disease pathogenesis and for testing potential therapies. TREX1/DNase III, an ER-associated exonuclease, is a negative regulator of innate immunity, and TREX1 represents one of the highest monogenic links to SLE. Curiously, most TREX1 mutations associated with SLE do not affect its DNase activity, but rather, disrupt its ER localization domain. The molecular cause for TREX1 SLE is unclear. We recently discovered a DNase- independent function of TREX1 that is required for regulating a novel ER-associated reaction. We also identified a previously unknown self-ligand derived from the ER in cells carrying TREX1 SLE mutations, as well as several small molecule inhibitors that target key enzymes that contribute to the immunopathogenesis of TREX1 SLE. Based on published and preliminary data, we hypothesize that TREX1 mutations with defective ER localization domain contribute to SLE by stimulating an ER-associated reaction that generates immunogenic self-ligands. Moreover, our biochemical data indicate that these self-ligands are free glycans. Therapeutic inhibition of this reaction or downstream innate immune signaling is predicted to ameliore TREX1 disease. We will elucidate this novel function of TREX1 with the following three specific aims.
Aim 1) Genetic and biochemical dissection of a novel ER-associated reaction activated by TREX1 SLE mutations, that causes release of free glycans. We will examine TREX1 SLE and other mutations using rescue assays established in preliminary studies to identify residues in TREX1 critically required for immune activation and free glycan release. We will also biochemically dissect the interaction between TREX1 and the ER-associated glycan catabolic reaction it regulates.
Aim 2) Determine how free glycan generated by TREX1 SLE mutants trigger autoimmunity. We have established an activity-based immune assay, which we will use to define structural details and immunogenic potential of free glycan as a self-ligand for TREX1 SLE and the corresponding immune signaling pathway.
Aim 3) Therapeutically diminish free glycan release or inhibit TBK1 signaling in Trex1-/- mice and TREX1 SLE mutant patient cells. We will further test our hypothesis in vivo by therapeutically targeting key enzymes implicated in TREX1 SLE pathogenesis. The significance of this proposal lies with the fact that it presents an important but previously unrecognized function of TREX1 as well as identification of free glycan as a novel self-ligand in SLE. This application will also reveal and test novel targets for the treatment of SLE through 're-purposing'existing compounds developed against key enzymes involved in TREX1 SLE.

Public Health Relevance

Despite significant progress in understanding human lupus, it remains elusive what could be the molecular cause of autoimmunity and how can we treat it. This project will investigate a novel function of an important gene, TREX1/DNASE III, which is strongly associated with SLE. Studies proposed here will elucidate the molecular mechanism leading to autoimmunity in TREX1 SLE, as well as develop an accelerated path to potential therapies.

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Research Project (R01)
Project #
1R01AR067135-01
Application #
8795632
Study Section
Hypersensitivity, Autoimmune, and Immune-mediated Diseases Study Section (HAI)
Program Officer
Mancini, Marie
Project Start
2014-09-16
Project End
2019-08-31
Budget Start
2014-09-16
Budget End
2015-08-31
Support Year
1
Fiscal Year
2014
Total Cost
$349,800
Indirect Cost
$129,800
Name
University of Texas Sw Medical Center Dallas
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
800771545
City
Dallas
State
TX
Country
United States
Zip Code
75390
Lu, Hua; Fermaintt, Charles S; Cherepanova, Natalia A et al. (2018) Mammalian STT3A/B oligosaccharyltransferases segregate N-glycosylation at the translocon from lipid-linked oligosaccharide hydrolysis. Proc Natl Acad Sci U S A 115:9557-9562
Yang, Kun; Huang, Ryan; Fujihira, Haruhiko et al. (2018) N-glycanase NGLY1 regulates mitochondrial homeostasis and inflammation through NRF1. J Exp Med 215:2600-2616
Yan, Nan (2017) Immune Diseases Associated with TREX1 and STING Dysfunction. J Interferon Cytokine Res 37:198-206
Kucej, Martin; Fermaintt, Charles S; Yang, Kun et al. (2017) Mitotic Phosphorylation of TREX1 C Terminus Disrupts TREX1 Regulation of the Oligosaccharyltransferase Complex. Cell Rep 18:2600-2607
Gonugunta, Vijay K; Sakai, Tomomi; Pokatayev, Vladislav et al. (2017) Trafficking-Mediated STING Degradation Requires Sorting to Acidified Endolysosomes and Can Be Targeted to Enhance Anti-tumor Response. Cell Rep 21:3234-3242
Sakai, Tomomi; Miyazaki, Takuya; Shin, Dong-Mi et al. (2017) DNase-active TREX1 frame-shift mutants induce serologic autoimmunity in mice. J Autoimmun 81:13-23
Warner, James D; Irizarry-Caro, Ricardo A; Bennion, Brock G et al. (2017) STING-associated vasculopathy develops independently of IRF3 in mice. J Exp Med 214:3279-3292
Pokatayev, Vladislav; Yan, Nan (2017) Methods of Assessing STING Activation and Trafficking. Methods Mol Biol 1656:167-174
Hasan, Maroof; Gonugunta, Vijay K; Dobbs, Nicole et al. (2017) Chronic innate immune activation of TBK1 suppresses mTORC1 activity and dysregulates cellular metabolism. Proc Natl Acad Sci U S A 114:746-751
Hasan, Maroof; Yan, Nan (2016) Therapeutic potential of targeting TBK1 in autoimmune diseases and interferonopathies. Pharmacol Res 111:336-342

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