Abstract

The conventional LPS or endotoxin preparations are heterogenous. Besides endotoxic components they contain non-toxic substances which are active in some of the beneficial effects attribute to endotoxins. These effects are immunopotentiation, enhanced proliferation of bone-marrow cells, resistance to infections, activation of the RES and increased resistance to a few experimental tumors. We found that this beneficial component (B component) of the commonly used LPS or ET preparation can be separated by chromatographic methods from the toxic component. Furthermore, we found that such B component can also be extracted directly from some rough mutant bacteria, from which the endotoxic components were previously removed.
The aim of this work is the characterization and chemical structural studies of the B component.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA024628-06
Application #
3166510
Study Section
Bacteriology and Mycology Subcommittee 1 (BM)
Project Start
1984-04-01
Project End
1987-09-30
Budget Start
1986-04-01
Budget End
1987-09-30
Support Year
6
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
University of Pennsylvania
Department
Type
Schools of Dentistry/Oral Hygn
DUNS #
042250712
City
Philadelphia
State
PA
Country
United States
Zip Code
19104

  Publications

Kovats, E; Somlyo, B; Csanky, E et al. (1992) Potentiation of HIV envelope glycoprotein and other immunogens by endotoxin (ET) and its molecular fragments. Int J Immunopharmacol 14:573-81
Keler, T; Smith, C A; Nowotny, A (1990) Chemistry and biology of a novel lipid contaminant of some endotoxin preparations with selective cytotoxicity to transformed cells. Adv Exp Med Biol 256:163-84
Somlyo, B; Kovats, E; Keler, T et al. (1990) Column liquid chromatography of endotoxins. J Chromatogr 525:329-38
Keler, T; Smith, C A (1989) Effects on growth and energy metabolism in untransformed and transformed BALB/c mouse fibroblasts by a novel cytotoxic compound. Cancer Res 49:7093-7
Nowotny, A; Keler, T; Pham, P H et al. (1988) Isolation of a nonendotoxic antitumor preparation from Serratia marcescens. J Biol Response Mod 7:296-308
Samu, J; Kovats, E; Nguyen, V et al. (1988) Thin-layer chromatography of endotoxins, their derivatives and contaminants. J Chromatogr 435:167-83
Keler, T; Kovats, E; Nguyen, V et al. (1987) Cytotoxicity of a novel lipid-like bacterial product. Biochem Biophys Res Commun 149:1033-41
Nowotny, A; Moore, M E; Nejman, G et al. (1987) Time dependency of endotoxin-induced resistance to transplantable tumors in mice. Cancer Invest 5:195-203
Keler, T; Nowotny, A (1986) Metachromatic assay for the quantitative determination of bacterial endotoxins. Anal Biochem 156:189-93
Rothman, J; Johnson, A G; Friedman, H et al. (1985) Biological effects of White-type polysaccharides of gram-negative bacteria. J Biol Response Mod 4:169-84