Abstract

It is planned to investigate the uvrA, uvrB and uvrC enzymes of E. coli which incise DNA irradiated with ultraviolet light.
We aim to purify uvrB protein and to identify and then purify uvrC protein. This will be done with the help of a plasmid carrying uvrB to increase the yield of the uvrB protein, and a lambda transducing phage carrying an amber mutant of uvrC to help in the identification, production and purification of the uvrC protein. Once the uvrC protein has been identified, we will investigate its properties and try to develop methods for its purification.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA026763-06
Application #
3167450
Study Section
Radiation Study Section (RAD)
Project Start
1980-02-01
Project End
1986-02-28
Budget Start
1985-03-01
Budget End
1986-02-28
Support Year
6
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Yale University
Department
Type
Schools of Medicine
DUNS #
082359691
City
New Haven
State
CT
Country
United States
Zip Code

  Publications

Akaboshi, E; Howard-Flanders, P (1990) RecA-ssDNA interaction: induced strand cleavage by hydroxyl radical at a defined distance from the 5' end. Mol Gen Genet 220:456-60
Sladek, F M; Melian, A; Howard-Flanders, P (1989) Incision by UvrABC excinuclease is a step in the path to mutagenesis by psoralen crosslinks in Escherichia coli. Proc Natl Acad Sci U S A 86:3982-6
Hahn, T R; West, S; Howard-Flanders, P (1988) RecA-mediated strand exchange reactions between duplex DNA molecules containing damaged bases, deletions, and insertions. J Biol Chem 263:7431-6
West, S C; Korner, A (1985) Cleavage of cruciform DNA structures by an activity from Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 82:6445-9