We have prepared genomic and cDNA clones including MHC gene products expressed in B cells, T cells, and teratocarcinoma cells. We will extend these genomic clones with the ultimate goal of obtaining the entire chromosomal region embedding the MHC complex in overlapping genomic clones. We have cloned and sequenced the genes for HLA-B7, HLA-A2 and another previously unidentified class I antigen, and the genes for HLA-DR and SB-alpha chain. We will characterize the DNA controlling the expression of gene products in different types of cells or cells at different stages of differentiation. We have constructed a range of recombinant HLA-B7 clones and are studying their expression in injected mouse embryos. (P)

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA030938-05
Application #
3169410
Study Section
Mammalian Genetics Study Section (MGN)
Project Start
1981-08-01
Project End
1986-05-31
Budget Start
1985-06-01
Budget End
1986-05-31
Support Year
5
Fiscal Year
1985
Total Cost
Indirect Cost
Name
Yale University
Department
Type
Schools of Medicine
DUNS #
082359691
City
New Haven
State
CT
Country
United States
Zip Code
Duceman, B W; Ness, D; Rende, R et al. (1986) HLA-JY328: mapping studies and expression of a polymorphic HLA class I gene. Immunogenetics 23:90-9