This revised grant application is focused on experimental studies to elucidate the biosynthesis of three biogenetically related families of natural products: (1) the Paraherquamides, Asperparalines, Malbranchemaides, Marcfortines and Chrysogenamide (the monooxopiperazines);(2) the Stephacidins, Notoamides, Waikialoids and Brevianamides (the dioxopiperazines);and (3) the Citrinadins, Citrinalins, Cyclopiamines and PF1270 alkaloids (the decarbonylated alkaloids). The goals are to exploit the powerful synergies of total synthesis, whole genome sequencing, bioinformatics analysis, genome mining, functional expression and X-ray crystallography of biosynthetic enzymes to fully elucidate the corresponding biosynthetic pathways to all three families of alkaloids. I. Paraherquamides, Asperparalines, Malbranchemaides, Marcfortines and Chrysogenamide: the Monooxopiperazines. Provocative evidence has been elucidated in our laboratory indicating that this class of alkaloids, are constructed by a rare biosynthetic intramolecular [4+2] cycloaddition reaction resulting from the reductive release of a Trp-Pro (or Trp-Pip) dipeptide amino-aldehyde from a NRPS module that upon reverse prenylation, suffers a cascade of cyclization, dehydration, tautomerization and intramolecular Diels-Alder cycloaddition to construct the monooxopiperazine bicyclo[2.2.2]diazaoctane ring system common to this family. Through the use of total chemical synthesis of isotopically labeled intermediate metabolites, genome mining, biosynthetic gene cluster identification and functional expression of biosynthetic enzymes, key features of the biosynthetic pathways to these complex secondary metabolites will be experimentally elucidated. In a multi-PI relationship and sub-award with Prof. David Sherman's laboratory (University of Michigan), we are actively engaged in the high-resolution elucidation of the entire biosynthetic pathway to these biomedically significant alkaloids. II. Stephacidins, Notoamides, Waikialoids and Brevianamides: The Dioxopiperazines. The dioxopiperazine family of bicyclo[2.2.2]diazaoctane alkaloids are constructed by a net oxidative transformation of a fully prenylated dioxopiperazine substrate. We have discovered that two orthologous species of Aspergillus produce the opposite enantiomers of Stephacidn A and Notoamide B. This fascinating enantiodivergent biogenesis will be further evaluated using bioinformatics analysis by a new collaborator, Prof. Martin Kreitman, a renowned evolutionary geneticist, to determine the evolutionary mechanisms that resulted in this rare production of opposite enantiomers of these complex alkaloids. III. Citrinadins, Citrinalins, Cyclopiamines and PF1270 Alkaloids: The Decarbonylated Alakloids. As a natural out-growth of our work on the Paraherquamide family of prenylated indole alkaloids, we propose to initiate a new project to study the total synthesis and biosynthesis of these structurally related alkaloids that appear to have arisen biogenetically from the reductive decarbonylation of bicyclo[2.2.2]diazaoctane progenitors. Here also, we shall deploy the powerful synergies of total synthesis, whole genome sequencing, bioinformatics analysis, genome mining and functional expression of biosynthetic enzymes to fully elucidate the corresponding biosynthetic pathways to all three families of alkaloids. In all three sub-projects, total synthesis of the natural products and isotopically-labeled biosynthetic intermediates and probe molecules will be utilized to confirm pathway transformations. New chemical entities generated in this program, either from the biological sources or through chemical synthesis, will be extensively screened and evaluated for biological activities at the Univ. of Michigan Center for Chemical Genomics, the National Human Genome Research Institute, and to Prof. Sachiko Tsukamoto (Japan) for analysis of biological activity using a series of biochemical and cell-based assays relevant to cancer and parasitic disease targets. Additional collaborators include: Prof. Sachiko Tsukamoto, Kumamoto University, Japan;Prof. Jens Frisvad, Technical University, Denmark;Prof. Martin Kreitman, University of Chicago;and Prof. Janet Smith, University of Michigan.

Public Health Relevance

The purpose of this application is to utilize the tools of chemical synthesis, whole genome sequencing, genome mining, biosynthetic gene cluster identification and functional expression of biosynthetic enzymes to study the molecular details of how Nature biosynthesizes biomedically significant natural substances that includes anti-cancer drugs. In addition, the chemical technologies being developed will be applied to the design and synthesis of new biologically active agents, with a focus on anti-cancer drugs, and anti-infective agents.

Agency
National Institute of Health (NIH)
Type
Research Project (R01)
Project #
2R01CA070375-18A1
Application #
8761664
Study Section
Synthetic and Biological Chemistry B Study Section (SBCB)
Program Officer
Fu, Yali
Project Start
Project End
Budget Start
Budget End
Support Year
18
Fiscal Year
2014
Total Cost
Indirect Cost
Name
Colorado State University-Fort Collins
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
City
Fort Collins
State
CO
Country
United States
Zip Code
80521
Welch, Timothy R; Williams, Robert M (2014) Epidithiodioxopiperazines. occurrence, synthesis and biogenesis. Nat Prod Rep 31:1376-404
Sunderhaus, James D; McAfoos, Timothy J; Finefield, Jennifer M et al. (2013) Synthesis and bioconversions of notoamide T: a biosynthetic precursor to stephacidin A and notoamide B. Org Lett 15:22-5
Guerra-Bubb, Jennifer; Croteau, Rodney; Williams, Robert M (2012) The early stages of taxol biosynthesis: an interim report on the synthesis and identification of early pathway metabolites. Nat Prod Rep 29:683-96
Li, Shengying; Finefield, Jennifer M; Sunderhaus, James D et al. (2012) Biochemical characterization of NotB as an FAD-dependent oxidase in the biosynthesis of notoamide indole alkaloids. J Am Chem Soc 134:788-91
Rath, Christopher M; Janto, Benjamin; Earl, Josh et al. (2011) Meta-omic characterization of the marine invertebrate microbial consortium that produces the chemotherapeutic natural product ET-743. ACS Chem Biol 6:1244-56
Finefield, Jennifer M; Sherman, David H; Tsukamoto, Sachiko et al. (2011) Studies on the biosynthesis of the notoamides: synthesis of an isotopomer of 6-hydroxydeoxybrevianamide E and biosynthetic incorporation into notoamide J. J Org Chem 76:5954-8
Finefield, Jennifer M; Greshock, Thomas J; Sherman, David H et al. (2011) Notoamide E: Biosynthetic incorporation into notoamides C and D in cultures of Aspergillus versicolor NRRL 35600. Tetrahedron Lett 52:1987-1989
Sunderhaus, James D; Sherman, David H; Williams, Robert M (2011) Studies on the Biosynthesis of the Stephacidin and Notoamide Natural Products: A Stereochemical and Genetic Conundrum. Isr J Chem 51:442-452
Williams, Robert M; Stille, J; Echavarren, A et al. (2011) Discussion Addendum for: 4-Methoxy-4'-nitrophenyl. Recent Advances in the Stille Biaryl Coupling Reaction and Applications in Complex Natural Products Synthesis. Organic Synth 88:197-201
Finefield, Jennifer M; Kato, Hikaru; Greshock, Thomas J et al. (2011) Biosynthetic studies of the notoamides: isotopic synthesis of stephacidin A and incorporation into notoamide B and sclerotiamide. Org Lett 13:3802-5

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