The general goal of this proposal is to develop non-intrusive optical spectroscopy as a real-time diagnostic technique for cervical dysplasia. In our previous study we have demonstrated good sensitivity and specificity of our fiber-optic probe for diagnosing HSIL in vivo and provided insight into the physical mechanisms which are the basis of our light scattering technique. In this work, we will build on our previous results, by modifying the fiber-optic probe and spectroscopy system to obtain better accuracy, developing a probe for the endocervical canal, studying the false positives and false negatives to understand the causes of inaccuracies, improving our understanding of the physical mechanisms that are the basis for the technique, and finally testing our diagnostic spectroscopic system in the clinic.

National Institute of Health (NIH)
National Cancer Institute (NCI)
Research Project (R01)
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Biomedical Imaging Technology Study Section (BMIT)
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Ossandon, Miguel
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Los Alamos National Lab
Los Alamos
United States
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Mourant, Judith R; Marina, Oana C; Hebert, Tiffany M et al. (2014) Hemoglobin parameters from diffuse reflectance data. J Biomed Opt 19:37004
Sanders, Claire K; Mourant, Judith R (2013) Advantages of full spectrum flow cytometry. J Biomed Opt 18:037004
Mourant, Judith R; Bocklage, Therese J; Powers, Tamara M et al. (2009) Detection of cervical intraepithelial neoplasias and cancers in cervical tissue by in vivo light scattering. J Low Genit Tract Dis 13:216-223
Mourant, Judith R; Powers, Tamara M; Bocklage, Therese J et al. (2009) In vivo light scattering for the detection of cancerous and precancerous lesions of the cervix. Appl Opt 48:D26-35
Mourant, Judith R; Bocklage, Therese J; Powers, Tamara M et al. (2007) In vivo light scattering measurements for detection of precancerous conditions of the cervix. Gynecol Oncol 105:439-45
Jiang, Yi; Pjesivac-Grbovic, Jelena; Cantrell, Charles et al. (2005) A multiscale model for avascular tumor growth. Biophys J 89:3884-94
LaRue, Karen E A; Khalil, Mona; Freyer, James P (2004) Microenvironmental regulation of proliferation in multicellular spheroids is mediated through differential expression of cyclin-dependent kinase inhibitors. Cancer Res 64:1621-31
Mourant, J R; Johnson, T M; Doddi, V et al. (2002) Angular dependent light scattering from multicellular spheroids. J Biomed Opt 7:93-9
Mourant, J R; Johnson, T M; Carpenter, S et al. (2002) Polarized angular dependent spectroscopy of epithelial cells and epithelial cell nuclei to determine the size scale of scattering structures. J Biomed Opt 7:378-87
Mourant, J R; Canpolat, M; Brocker, C et al. (2000) Light scattering from cells: the contribution of the nucleus and the effects of proliferative status. J Biomed Opt 5:131-7

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