Prostate cancer (PCa) is the leading cancer diagnosed and the second cause of cancer death in American men. Currently, therapeutic strategies for the treatment of hormone-independent metastatic PCa are very limited. The long-term goal of our competitive renewal application is to determine whether and how transforming growth factor beta (TGF?) signaling pathway can be targeted for the development of novel therapeutic approaches to inhibit PCa growth and metastasis in preclinical animal models. In our preliminary studies, we found that systemic administration of various TGF? antagonists inhibited the growth and angiogenesis of xenograft prostate tumors and metastasis of orthotopic prostate tumors. The inhibition of prostate tumor progression by TGF? antagonists was in part due to the blockade of TGF? signaling in tumor-associated stromal cells because partial blockade of TGF? signaling by the expression of a dominant-negative receptor in prostate stromal cells significantly attenuated their tumor-promoting activity. Thus, our central hypothesis in the competitive renewal application is that blockade of TGF? signaling in prostate stromal cells may be developed as a novel strategy to inhibit PCa growth and metastasis. In our specific aim 1, we will employ a tumor and stromal cell co-transplantation system to determine the role of TGF? signaling in prostate stromal cells in the regulation of tumor-stroma crosstalk and tumor progression. During the past funding cycle, we developed a novel TGF? -binding protein that can act as a pan- TGF? antagonist. We have also developed a novel androgen-receptor positive human PCa cell line that induces osteoblastic bone metastasis when inoculated intracardiacally in mice. In our specific aim 2, we will compare the efficacy of the novel pan-TGF? binding protein with that of a small TGF? type I receptor kinase inhibitor in the inhibition of growth and metastasis of the novel PCa cell line as well as other PCa cells in xenograft and orthotopic PCa models and the spontaneous PCa model of TRAMP mice. In the specific aim 3, we will test the novel hypothesis that the combination of a TGF? antagonist and a chemotherapeutic drug will produce a synergistic inhibitory effect on PCa growth and metastasis in xenograft and orthotopic PCa models. Our proposed research will reveal whether targeting TGF? signaling in tumor-associated stromal cells is feasible as a novel therapeutic approach to inhibit PCa growth and metastasis in a preclinical setting. The knowledge gained from our research may lay the foundation for clinical trials with TGF? antagonist alone or in combination with a chemotherapeutic agent for the treatment of metastatic PCa. Project Narrative Prostate cancer is a major health issue in the aging population of American men. Prostate cancer progression is associated with over-production of transforming growth factor beta (TGF?). Our preliminary studies have shown that TGF? can drive hormone-independent prostate cancer progression and metastasis. Our proposed research will determine whether systemic treatment with TGF? antagonists is of therapeutic potential for the inhibition of prostate tumor growth and bone metastasis in mouse models of prostate cancer. The knowledge gained from our research may lay the foundation for clinical trials with TGF? antagonist alone or in combination with a chemotherapeutic agent for the treatment of metastatic PCa.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA079683-13
Application #
8242804
Study Section
Basic Mechanisms of Cancer Therapeutics Study Section (BMCT)
Program Officer
Muszynski, Karen
Project Start
1999-01-01
Project End
2014-03-31
Budget Start
2012-04-01
Budget End
2014-03-31
Support Year
13
Fiscal Year
2012
Total Cost
$215,114
Indirect Cost
$70,256
Name
University of Texas Health Science Center San Antonio
Department
Biology
Type
Schools of Medicine
DUNS #
800772162
City
San Antonio
State
TX
Country
United States
Zip Code
78229
Mishra, Sweta; Lin, Chun-Lin; Huang, Tim H-M et al. (2014) MicroRNA-21 inhibits p57Kip2 expression in prostate cancer. Mol Cancer 13:212
Biswas, Tanuka; Gu, Xiang; Yang, Junhua et al. (2014) Attenuation of TGF-* signaling supports tumor progression of a mesenchymal-like mammary tumor cell line in a syngeneic murine model. Cancer Lett 346:129-38
Wang, Danhan; Gao, Hui; Bandyopadhyay, Abhik et al. (2014) Pubertal bisphenol A exposure alters murine mammary stem cell function leading to early neoplasia in regenerated glands. Cancer Prev Res (Phila) 7:445-55
Mishra, S; Deng, J J; Gowda, P S et al. (2014) Androgen receptor and microRNA-21 axis downregulates transforming growth factor beta receptor II (TGFBR2) expression in prostate cancer. Oncogene 33:4097-106
Gowda, Pramod S; Deng, Jianhong D; Mishra, Sweta et al. (2013) Inhibition of hedgehog and androgen receptor signaling pathways produced synergistic suppression of castration-resistant prostate cancer progression. Mol Cancer Res 11:1448-61
Mu, Xiaoxin; Lin, Shu; Yang, Junhua et al. (2013) TGF-* signaling is often attenuated during hepatotumorigenesis, but is retained for the malignancy of hepatocellular carcinoma cells. PLoS One 8:e63436
Lin, Shu; Yang, Junhua; Elkahloun, Abdel G et al. (2012) Attenuation of TGF-* signaling suppresses premature senescence in a p21-dependent manner and promotes oncogenic Ras-mediated metastatic transformation in human mammary epithelial cells. Mol Biol Cell 23:1569-81
Mukhopadhyay, Keya De; Bandyopadhyay, Abhik; Chang, Ting-Tung A et al. (2011) Isolation and characterization of a metastatic hybrid cell line generated by ER negative and ER positive breast cancer cells in mouse bone marrow. PLoS One 6:e20473
Lin, Shu; Yu, Lan; Yang, Junhua et al. (2011) Mutant p53 disrupts role of ShcA protein in balancing Smad protein-dependent and -independent signaling activity of transforming growth factor-? (TGF-?). J Biol Chem 286:44023-34
Sudarshan, Sunil; Shanmugasundaram, Karthigayan; Naylor, Susan L et al. (2011) Reduced expression of fumarate hydratase in clear cell renal cancer mediates HIF-2? accumulation and promotes migration and invasion. PLoS One 6:e21037

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