Collagen serves as a structural scaffold and a barrier between tissues, and thus collagen catabolism (collagenolysis) is required to be a tightly regulated process in normal physiology. In turn, the destruction or damage of collagen during pathological states plays a role in tumor growth and invasion, cartilage degradation, or atherosclerotic plaque formation and rupture. Only a small number of proteases have been identified capable of efficient processing of triple-helical regions of collagens. Several members of the zinc metalloenzyme family, specifically matrix metalloproteinases (MMPs), possess collagenolytic activity. A mechanistic understanding of the cleavage of intact collagens has been pursued for many years;the results of such studies could lead to the development of truly selective MMP inhibitors. Our laboratory developed triple-helical peptides (THPs) as MMP substrates, with the goal of using these models to dissect collagenolytic behavior. Our work with THP substrates, along with prior studies from other research groups, have led to a "conformational entropy shift" hypothesis explaining how MMPs process collagen without input from an external energy source. The research plan described herein focuses on testing our collagenolysis hypothesis by utilizing several biophysical approaches [NMR spectroscopy, hydrogen/deuterium exchange mass spectrometry (HDX MS), X-ray crystallography, and X-ray absorption spectroscopy (XAS)] in combination with site-specific mutagenesis and kinetic analyses to precisely determinate the roles of MMP regions and residues in the binding, unwinding, and hydrolysis of triple-helical structures. Variants of THPs will be created, by site-directed and combinatorial approaches, to obtain substrates that are selective within the collagenolytic MMPs. Based on the mechanistic results, we will compare the inhibitory capabilities of phosphinate-containing THPs using site-directed and combinatorial libraries to develop novel, selective MMP inhibitors. Co-crystallization and HDX MS of MMPs and THP inhibitors will be utilized to evaluate the sites of interaction between the two biomolecules, allowing for further optimization of lead compounds. Select inhibitors will be tested using cells overexpressing the targeted MMPs, in a model of angiogenesis, and in mouse models of breast carcinoma and melanoma. Ultimately, we would like to obtain inhibitors that target those proteases implicated in cancer progression (MMP-2, MMP-9, and MT1-MMP) while sparing proteases with host-beneficial functions (MMP-3 and MMP- 8). PUBLIC HEALTH REVELANCE: The present study is designed to create a novel class of therapeutic agents to selectively stop the action of tumor-associated enzymes that degrade proteins (proteases). These proteases have been shown to be important for cancer progression, and thus blocking their function will impair the spread of cancer.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA098799-11
Application #
8232022
Study Section
Drug Discovery and Molecular Pharmacology Study Section (DMP)
Program Officer
Knowlton, John R
Project Start
2003-03-05
Project End
2013-02-28
Budget Start
2012-03-01
Budget End
2013-02-28
Support Year
11
Fiscal Year
2012
Total Cost
$323,704
Indirect Cost
$111,731
Name
Torrey Pines Institute for Molecular Studies
Department
Type
DUNS #
605758754
City
Port Saint Lucie
State
FL
Country
United States
Zip Code
34987
Knapinska, Anna M; Amar, Sabrina; He, Zhong et al. (2016) Matrix metalloproteinases as reagents for cell isolation. Enzyme Microb Technol 93-94:29-43
Prior, Stephen H; Byrne, Todd S; Tokmina-Roszyk, Dorota et al. (2016) Path to Collagenolysis: COLLAGEN V TRIPLE-HELIX MODEL BOUND PRODUCTIVELY AND IN ENCOUNTERS BY MATRIX METALLOPROTEINASE-12. J Biol Chem 291:7888-901
Fields, Gregg B; Stawikowski, Maciej J (2016) Imaging Matrix Metalloproteinase Activity Implicated in Breast Cancer Progression. Methods Mol Biol 1406:303-29
Singh, Warispreet; Fields, Gregg B; Christov, Christo Z et al. (2016) Importance of the Linker Region in Matrix Metalloproteinase-1 Domain Interactions. RSC Adv 6:23223-23232
Singh, Warispreet; Fields, Gregg B; Christov, Christo Z et al. (2016) Effects of Mutations on Structure-Function Relationships of Matrix Metalloproteinase-1. Int J Mol Sci 17:
Cerofolini, Linda; Amar, Sabrina; Lauer, Janelle L et al. (2016) Bilayer Membrane Modulation of Membrane Type 1 Matrix Metalloproteinase (MT1-MMP) Structure and Proteolytic Activity. Sci Rep 6:29511
Gao, Yu; Amar, Sabrina; Pahwa, Sonia et al. (2015) Rapid lead discovery through iterative screening of one bead one compound libraries. ACS Comb Sci 17:49-59
Van Doren, Steven R (2015) Matrix metalloproteinase interactions with collagen and elastin. Matrix Biol 44-46:224-31
Knapinska, Anna M; Tokmina-Roszyk, Dorota; Amar, Sabrina et al. (2015) Solid-phase synthesis, characterization, and cellular activities of collagen-model Nanodiamond-peptide conjugates. Biopolymers 104:186-95
Amar, Sabrina; Fields, Gregg B (2015) Potential clinical implications of recent matrix metalloproteinase inhibitor design strategies. Expert Rev Proteomics 12:445-7

Showing the most recent 10 out of 84 publications