The objective of this research is to further develop and clinically validate a real-time multispectral confocal microendoscope for in vivo diagnosis of ovarian cancer. The confocal microendoscope is a new type of instrument for visualizing tissue at the cellular level and has shown great promise for performing optical biopsy. Confocal microendoscopy has the potential to provide a physician with an immediate evaluation of tissue and to survey a much greater area of tissue, reducing the sampling error of traditional tissue extraction biopsy. A confocal microlaparoscope system was constructed and tested in vivo in humans during the prior funding period.
A specific aim of this work is to now validate the system and show that it can be used to detect cancer of the ovary during a laparoscopic procedure. Additional objectives of the work are to expand the use of the instrument to the detection of peritoneal implants of ovarian cancer throughout the abdominal cavity and to develop and test a system capable of imaging inside the fallopian tubes. Further technical development is aimed at improving the confocal imaging performance and adding optical coherence tomography as a complimentary imaging modality. The new instrument will incorporate the two imaging modalities into a single compact probe with seamless and rapid switching between modes of operation. In addition to the technology development and clinical translation, another aim of the project is to develop and test targeted contrast agents that provide safer and more effective in vivo identification of ovarian cancer.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA115780-07
Application #
8257546
Study Section
Biomedical Imaging Technology Study Section (BMIT)
Program Officer
Zhang, Yantian
Project Start
2005-09-01
Project End
2014-04-30
Budget Start
2012-05-01
Budget End
2013-04-30
Support Year
7
Fiscal Year
2012
Total Cost
$276,708
Indirect Cost
$89,715
Name
University of Arizona
Department
Radiation-Diagnostic/Oncology
Type
Schools of Medicine
DUNS #
806345617
City
Tucson
State
AZ
Country
United States
Zip Code
85721
Risi, Matthew D; Rouse, Andrew R; Chambers, Setsuko K et al. (2016) Pilot Clinical Evaluation of a Confocal Microlaparoscope for Ovarian Cancer Detection. Int J Gynecol Cancer 26:248-54
Risi, Matthew D; Makhlouf, Houssine; Rouse, Andrew R et al. (2015) Analysis of multimode fiber bundles for endoscopic spectral-domain optical coherence tomography. Appl Opt 54:101-13
Wu, Tzu-Yu; Rouse, Andrew R; Chambers, Setsuko K et al. (2014) Confocal microlaparoscope for imaging the fallopian tube. J Biomed Opt 19:116010
Risi, Matthew D; Makhlouf, Houssine; Rouse, Andrew R et al. (2014) Design and Performance of a Multi-Point Scan Confocal Microendoscope. Photonics 1:421-431
Kano, Angelique; Rouse, Andrew R; Gmitro, Arthur F (2013) Ultrathin single-channel fiberscopes for biomedical imaging. J Biomed Opt 18:16013
Leung, Hui Min; Schafer, Rachel; Pagel, Mark M et al. (2013) Multimodality pH imaging in a mouse dorsal skin fold window chamber model. Proc SPIE Int Soc Opt Eng 8574:
Tanbakuchi, Anthony A; Udovich, Joshua A; Rouse, Andrew R et al. (2010) In vivo imaging of ovarian tissue using a novel confocal microlaparoscope. Am J Obstet Gynecol 202:90.e1-9
Tanbakuchi, Anthony A; Rouse, Andrew R; Udovich, Joshua A et al. (2009) Clinical confocal microlaparoscope for real-time in vivo optical biopsies. J Biomed Opt 14:044030
Tanbakuchi, Anthony A; Rouse, Andrew R; Gmitro, Arthur F (2009) Monte Carlo characterization of parallelized fluorescence confocal systems imaging in turbid media. J Biomed Opt 14:044024
Udovich, J A; Besselsen, D G; Gmitro, A F (2009) Assessment of acridine orange and SYTO 16 for in vivo imaging of the peritoneal tissues in mice. J Microsc 234:124-9

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