The long-term goal of this research is to develop ultra-sensitive magnetic resonance imaging (MRI) probes that can provide cancer-specific detection of lung tumors by MRI. MRI is a clinical imaging technique that has broad applications in non-invasive diagnosis and post-therapy assessment for cancer. Although Gd-DTPA (Magnevist.) and other small molecular weight agents work well for dynamic contrast enhancement MRI applications, these agents are not very sensitive (lower limit of detection is ~10-4 M) and therefore cannot be used for detecting specific biological markers in vivo. In this application, we will investigate the use of superparamagnetic polymeric micelles (SPPM) that are loaded with a cluster of magnetite nanoparticles for molecular imaging of lung cancer. This platform demonstrated ~20 pM sensitivity of detection by MRI that will be essential for diagnosing lung cancers. A novel class of lung cancer-targeting peptides (LCPs) identified from phage screening will be functionalized on the surface of SPPM to target lung cancer cells. The isolated peptides demonstrated remarkable binding affinities ( A combination of novel MRI imaging method and ultra-sensitive molecular probes will be developed to provide cell-specific characterization of lung tumors. This knowledge can facilitate timely intervention of lung cancer to achieve personalized medicine.
Public Health Relevance
A combination of novel MRI imaging method and ultra-sensitive molecular probes will be developed to provide cell-specific characterization of lung tumors. This knowledge can facilitate timely intervention of lung cancer to achieve personalized medicine.
|Luo, Min; Wang, Hua; Wang, Zhaohui et al. (2017) A STING-activating nanovaccine for cancer immunotherapy. Nat Nanotechnol 12:648-654|
|Wang, Chensu; Wang, Yiguang; Li, Yang et al. (2015) A nanobuffer reporter library for fine-scale imaging and perturbation of endocytic organelles. Nat Commun 6:8524|
|Wang, Yiguang; Zhou, Kejin; Huang, Gang et al. (2014) A nanoparticle-based strategy for the imaging of a broad range of tumours by nonlinear amplification of microenvironment signals. Nat Mater 13:204-12|
|Huang, Gang; Chen, Huabing; Dong, Ying et al. (2013) Superparamagnetic iron oxide nanoparticles: amplifying ROS stress to improve anticancer drug efficacy. Theranostics 3:116-26|
|Zhang, Shanrong; Zhou, Kejin; Huang, Gang et al. (2013) A novel class of polymeric pH-responsive MRI CEST agents. Chem Commun (Camb) 49:6418-20|
|Huang, Xiaonan; Huang, Gang; Zhang, Shanrong et al. (2013) Multi-chromatic pH-activatable 19F-MRI nanoprobes with binary ON/OFF pH transitions and chemical-shift barcodes. Angew Chem Int Ed Engl 52:8074-8|
|Togao, Osamu; Kessinger, Chase W; Huang, Gang et al. (2013) Characterization of lung cancer by amide proton transfer (APT) imaging: an in-vivo study in an orthotopic mouse model. PLoS One 8:e77019|
|Yu, Haijun; Zou, Yonglong; Wang, Yiguang et al. (2011) Overcoming endosomal barrier by amphotericin B-loaded dual pH-responsive PDMA-b-PDPA micelleplexes for siRNA delivery. ACS Nano 5:9246-55|
|Ding, Huiying; Mora, Ruben; Gao, Jinming et al. (2011) Characterization and optimization of mTHPP nanoparticles for photodynamic therapy of head and neck cancer. Otolaryngol Head Neck Surg 145:612-7|
|Doi, Shigehiro; Zou, Yonglong; Togao, Osamu et al. (2011) Klotho inhibits transforming growth factor-beta1 (TGF-beta1) signaling and suppresses renal fibrosis and cancer metastasis in mice. J Biol Chem 286:8655-65|
Showing the most recent 10 out of 20 publications