It is widely believed that the greatest achievement that can be made in cancer management is the early detection and subsequent treatment of disease. The next generation cancer management strategies will greatly benefit from technologies that combine imaging, targeting, and treating of the earliest stage disease. Recent advances in nanotechnologies have produced a class of optically active metal particles with highly desirable molecular and optical properties suitable for combining these three aspects. They can be imaged optically as they exhibit strong optical absorption that is tunable to the near infrared spectrum where in vivo optical imaging is optimum. As they are fabricated from noble metals, they are biologically non-reactive and non- toxic, and they have surface chemistries that are conducive to antibody labeling. Their metal structure is extremely stable and efficiently converts absorbed light to heat, allowing for photothermal therapeutic applications. Successful translation of these nanoparticles as combined imaging, targeting, and therapeutic agents requires the development of non-invasive imaging strategies, in vivo cell specific targeting protocols, and optimum dosimetry planning. The proposed project represents a long term interdisciplinary effort to develop a technology combining imaging, targeting, and treatment of cancer using metal nanoparticles as the mediator. The proposed studies will develop minimally invasive optical imaging and sensing tools for quantitative assessment of nanoparticle location, tissue pathology, and therapeutic response (Aim 1). We will investigate the kinetics of nanoparticle tumor targeting and develop strategies for efficient delivery nanoparticles to the tumor site (Aim 2). We will determine the optimum laser irradiation of the nanoparticle labeled tumor to induce cell injury at the single cell level. Our approach includes multiscale molecular modeling of protein-nanoparticle interactions and in vitro verification (Aim 3). The final outcome of this project will demonstrate in a preclinical animal model highly selective cancer cell killing without damaging immediately adjacent normal cells (Aim 4). In the long term, such technologies offer the possibility to circumvent current limitations of surgical removal by selectively treating disease at the cellular and sub-cellular level. It is widely believed that the greatest achievement that can be made in cancer management is the early detection and subsequent treatment of disease. The proposed project represents an interdisciplinary effort to develop a technology combining imaging, targeting, and treatment of cancer using metal nanoparticles as the mediator. In the long term, such technologies offer the possibility to circumvent current limitations of surgical removal by selectively treating disease at the cellular and sub-cellular level. It is widely believed that the greatest achievement that can be made in cancer management is the early detection and subsequent treatment of disease. The proposed project represents an interdisciplinary effort to develop a technology combining imaging, targeting, and treatment of cancer using metal nanoparticles as the mediator. In the long term, such technologies offer the possibility to circumvent current limitations of surgical removal by selectively treating disease at the cellular and sub-cellular level.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA132032-05
Application #
8223310
Study Section
Special Emphasis Panel (ZRG1-BST-M (50))
Program Officer
Tandon, Pushpa
Project Start
2008-05-05
Project End
2014-02-28
Budget Start
2012-03-01
Budget End
2014-02-28
Support Year
5
Fiscal Year
2012
Total Cost
$291,182
Indirect Cost
$63,387
Name
University of Texas Austin
Department
Biomedical Engineering
Type
Schools of Engineering
DUNS #
170230239
City
Austin
State
TX
Country
United States
Zip Code
78712
Lee, Jihyoun; Chatterjee, Dev Kumar; Lee, Min Hyuk et al. (2014) Gold nanoparticles in breast cancer treatment: promise and potential pitfalls. Cancer Lett 347:46-53
Yang, Bin; Sharma, Manu; Tunnell, James W (2013) Attenuation-corrected fluorescence extraction for image-guided surgery in spatial frequency domain. J Biomed Opt 18:80503
Hennessy, Ricky; Lim, Sam L; Markey, Mia K et al. (2013) Monte Carlo lookup table-based inverse model for extracting optical properties from tissue-simulating phantoms using diffuse reflectance spectroscopy. J Biomed Opt 18:037003
Chatterjee, Dev Kumar; Wolfe, Tatiana; Lee, Jihyoun et al. (2013) Convergence of nanotechnology with radiation therapy-insights and implications for clinical translation. Transl Cancer Res 2:256-268
Panthani, Matthew G; Khan, Tarik A; Reid, Dariya K et al. (2013) In vivo whole animal fluorescence imaging of a microparticle-based oral vaccine containing (CuInSe(x)S(2-x))/ZnS core/shell quantum dots. Nano Lett 13:4294-8
Yu, Yixuan; Hessel, Colin M; Bogart, Timothy D et al. (2013) Room temperature hydrosilylation of silicon nanocrystals with bifunctional terminal alkenes. Langmuir 29:1533-40
Rasch, Michael R; Bosoy, Christian A; Yu, Yixuan et al. (2012) Chains, sheets, and droplets: assemblies of hydrophobic gold nanocrystals with saturated phosphatidylcholine lipid and squalene. Langmuir 28:15160-7
Pattani, Varun P; Tunnell, James W (2012) Nanoparticle-mediated photothermal therapy: a comparative study of heating for different particle types. Lasers Surg Med 44:675-84
Nichols, Brandon S; Rajaram, Narasimhan; Tunnell, James W (2012) Performance of a lookup table-based approach for measuring tissue optical properties with diffuse optical spectroscopy. J Biomed Opt 17:057001
Puvanakrishnan, Priyaveena; Diagaradjane, Parmeswaran; Kazmi, S M Shams et al. (2012) Narrow band imaging of squamous cell carcinoma tumors using topically delivered anti-EGFR antibody conjugated gold nanorods. Lasers Surg Med 44:310-7

Showing the most recent 10 out of 28 publications