The goal of this academic-industrial partnership program is to develop a turn-key, wavelength tunable, all-fiber, energetic femtosecond sources for cancer detection and for guiding of tissue biopsy and surgery. The proposed research program consists of two components: (1) engineering development of the optical fiber and the femtosecond sources;and (2) medical validation of the all-fiber femtosecond sources, when coupled with the existing multiphoton microscope and newly developed multiphoton medical endoscope, for cancer research and cancer detection. The engineering component involves close collaboration between Cornell University and our industrial partner OFS Laboratories, with design input from the collaborating biomedical researchers and practicing oncologists. The medical validation component leverages our existing development of multiphoton microscopy and endoscopy for cancer research. Experiments of in vivo animal imaging and spectroscopy will be performed at Cornell Ithaca Campus. Medical validation experiments will also be carried out at Cornell Weill Medical College in ex vivo human cancer after it has been removed surgically from the patient. The three specific aims are: 1. Design and fabricate novel HOM fiber modules for SSFS at input wavelengths of 1030 nm and 775 nm. 2. Demonstrate two all-fiber femtosecond sources with wavelength tuning ranges of (1) 775 nm to 1000 nm and (2) 1030 nm to 1300 nm. The output pulse energies will be first at 2 nJ and then at 5 to 10 nJ. We will then Integrate the all-fiber source with in-house developed multiphoton microscopes and endoscopes. 3. Medical validation of the new femtosecond sources, coupled to the micro/endoscopes, for multiphoton spectroscopy, endoscopy, and microscopy in cancer research and diagnostics. Our overall goal by the end of the grant period is to have a prototype all-fiber, turn-key, wavelength tunable, femtosecond laser, coupled seamlessly to a medical multiphoton micro/endoscope, and medically validated in in vivo imaging of animal cancer model and ex vivo imaging of human cancer. The successful completion of this research program will undoubtedly benefit the multiphoton community in general, and enable multiphoton in vivo imaging in clinical applications. The proposed program, if successfully completed, leads to all-fiber, wavelength tunable, energetic femtosecond sources that will have a broad impact on multiphoton biomedical imaging. There are significant practical advantages offered by the all-fiber configuration, such as compact foot print, robust operation, and operational safety in a clinical environment. The successful completion of this research program will undoubtedly benefit the multiphoton community in general, and enable multiphoton in vivo imaging in clinical applications.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA133148-03
Application #
7843491
Study Section
Special Emphasis Panel (ZRG1-SBIB-S (50))
Program Officer
Baker, Houston
Project Start
2008-08-04
Project End
2012-05-31
Budget Start
2010-06-01
Budget End
2011-05-31
Support Year
3
Fiscal Year
2010
Total Cost
$431,379
Indirect Cost
Name
Cornell University
Department
Engineering (All Types)
Type
Schools of Engineering
DUNS #
872612445
City
Ithaca
State
NY
Country
United States
Zip Code
14850
Horton, Nicholas G; Wang, Ke; Kobat, Demirhan et al. (2013) In vivo three-photon microscopy of subcortical structures within an intact mouse brain. Nat Photonics 7:
Ouzounov, Dimitre G; Rivera, David R; Williams, Wendy O et al. (2013) Dual modality endomicroscope with optical zoom capability. Biomed Opt Express 4:1494-503
Ouzounov, Dimitre G; Rivera, David R; Webb, Watt W et al. (2013) Miniature varifocal objective lens for endomicroscopy. Opt Lett 38:3103-6
Guo, Hengchang; Aleyasin, Hossein; Howard, Scott S et al. (2013) Two-photon fluorescence imaging of intracellular hydrogen peroxide with chemoselective fluorescent probes. J Biomed Opt 18:106002
Howard, Scott S; Straub, Adam; Horton, Nicholas et al. (2013) Frequency Multiplexed In Vivo Multiphoton Phosphorescence Lifetime Microscopy. Nat Photonics 7:33-37
Xu, C; Wise, F W (2013) Recent Advances in Fiber Lasers for Nonlinear Microscopy. Nat Photonics 7:
Wang, Ke; Zhang, Delong; Charan, Kriti et al. (2013) Time-lens based hyperspectral stimulated Raman scattering imaging and quantitative spectral analysis. J Biophotonics 6:815-20
Brown, Christopher M; Rivera, David R; Pavlova, Ina et al. (2012) In vivo imaging of unstained tissues using a compact and flexible multiphoton microendoscope. J Biomed Opt 17:040505
Cheng, Ji; Pedersen, Martin E V; Charan, Kriti et al. (2012) Intermodal Cerenkov radiation in a higher-order-mode fiber. Opt Lett 37:4410-2
Rivera, David R; Brown, Christopher M; Ouzounov, Dimitre G et al. (2012) Multifocal multiphoton endoscope. Opt Lett 37:1349-51

Showing the most recent 10 out of 17 publications