Pre-B cells in human bone marrow are destined to die unless they are rescued through survival signals from a successfully assembled pre-B cell receptor (pre-BCR). Congenital defects in pre-BCR-related signaling molecules cause a severe block of early B cell development in humans. Likewise, B cell lineage acute lymphoblastic leukemia (ALL) cells are arrested at early stages of B cell development. B cell lineage ALL represents by far the most frequent malignancy in children and is also common in adults. Despite significant advances over the past four decades, cytotoxic treatment strategies have recently reached a plateau with cure rates at ~80 percent for children and 55 percent for adults. Relapse after cytotoxic drug treatment, initial drug-resistance and dose-limiting toxicity are among the most frequent complications of current therapy approaches. For this reason, pathway-specific treatment strategies seem promising to further improve therapy options for ALL patients. The following key observations during the initial project period lay the foundation for this renewal application: ? ALL can be subdivided into two groups that fundamentally differ with respect to pre-BCR function. In TCF3- rearranged ALL, pre-BCR signaling enables (Type 1), but suppresses (Type 2) leukemic transformation in other cytogenetic (e.g. MLL-rearranged, Ph+) ALL subtypes (Trageser et al., J Exp Med 2009). ? The divergent outcome in Type 1 and Type 2 ALL is mirrored by contrasting functions of pre-BCR signaling at specific stages of normal B cell development: The pre-BCR drives proliferation of Hardy Fraction C'but differentiation and cell cycle arrest in Fraction D pre-B cells (Nahar et al., Blood 2011). ? In Fraction C'pre-B cells and Type 1 ALL cells, pre-BCR signaling promotes survival signaling by activation of SOX4 (Ramezani et al., Blood 2013), whereas BACH2 mediates negative selection at the pre-BCR checkpoint and tumor suppression in ALL cells (Swaminathan et al., Nature Medicine 2013). ? In Fraction D pre-B cells that passed the pre-BCR checkpoint, BCL6 mediates survival (Duy et al., J Exp Med 2010). Likewise, BCL6 promotes survival and a previously unrecognized form of drug-resistance in Ph+ ALL (Duy et al., Nature 2011) and self-renewal of leukemia-initiating cells in Ph+ ALL (Hurtz et al., J Exp Med 2011). Based on these and other findings, we propose three Aims for the second project period, namely to understand the mechanistic switch of pre-BCR function from proliferation to differentiation/cell cycle arrest (Aim 1), to validate pre- BCR checkpoint regulators as potential therapeutic targets in genetic loss-of-function models (Aim 2) and to leverage this information to develop a strategy for pharmacological targeting of the pre-BCR pathway and to develop biomarkers that predict outcomes and facilitate risk stratification for patients with ALL (Aim 3). The central goal of this proposal is to establish the role of pre-BCR signaling during malignant transformation and clonal evolution of ALL and to target individual components of its signaling cascade for the development of novel pathway-specific therapy approaches for ALL.

Public Health Relevance

The proposed studies are of immediate relevance to public health, because data from four major clinical trials in 2013 demonstrated that targeting of the (pre-) B cell receptor tyrosine kinases SYK and BTK achieves durable clinical responses in various mature B cell malignancies. The four 2013 clinical studies were based on clinical use of the (pre-) B cell receptor tyrosine kinase inhibitors Ibrutinib, and Fostamatinib (NCT00446095;NCT01796470;NCT01105247;NCT00849654). Despite the critical role of pre-BCR signaling in ALL, the clinical successes of Ibrutinib (BTK) and Fostamatinib (SYK) in mature B cell lymphoma could not be replicated in pre-clinical models for B cell lineage ALL. While ALL cells from some patients exhibit extremely high sensitivity to BTK/SYK inhibition, ALL cells from other patients are completetly resistant to Ibrutinib (BTK) and Fostamatinib/GS-9973 (SYK). These findings suggest that critical additional information on pathway-specific targeting of pre-BCR signaling molecules is needed to effectively use these and other agents in the treatment of B cell lineage ALL.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
2R01CA137060-06
Application #
8696722
Study Section
Tumor Cell Biology Study Section (TCB)
Program Officer
Howcroft, Thomas K
Project Start
2008-12-01
Project End
2019-01-31
Budget Start
2014-02-20
Budget End
2015-01-31
Support Year
6
Fiscal Year
2014
Total Cost
$298,146
Indirect Cost
$108,947
Name
University of California San Francisco
Department
Pathology
Type
Schools of Medicine
DUNS #
094878337
City
San Francisco
State
CA
Country
United States
Zip Code
94143
Shojaee, Seyedmehdi; Chan, Lai N; Buchner, Maike et al. (2016) PTEN opposes negative selection and enables oncogenic transformation of pre-B cells. Nat Med 22:379-87
Lu, Zhengfei; Lieber, Michael R; Tsai, Albert G et al. (2015) Human lymphoid translocation fragile zones are hypomethylated and have accessible chromatin. Mol Cell Biol 35:1209-22
Chang, Bill H; Johnson, Kara; LaTocha, Dorian et al. (2015) YM155 potently kills acute lymphoblastic leukemia cells through activation of the DNA damage pathway. J Hematol Oncol 8:39
Swaminathan, Srividya; Klemm, Lars; Park, Eugene et al. (2015) Mechanisms of clonal evolution in childhood acute lymphoblastic leukemia. Nat Immunol 16:766-74
Shojaee, Seyedmehdi; Caeser, Rebecca; Buchner, Maike et al. (2015) Erk Negative Feedback Control Enables Pre-B Cell Transformation and Represents a Therapeutic Target in Acute Lymphoblastic Leukemia. Cancer Cell 28:114-28
Greaves, Mel; Müschen, Markus (2015) Infection and the Perils of B-cell Activation. Cancer Discov 5:1244-6
Montamat-Sicotte, Damien; Litzler, Ludivine C; Abreu, Cecilia et al. (2015) HSP90 inhibitors decrease AID levels and activity in mice and in human cells. Eur J Immunol 45:2365-76
Geng, Huimin; Hurtz, Christian; Lenz, Kyle B et al. (2015) Self-enforcing feedback activation between BCL6 and pre-B cell receptor signaling defines a distinct subtype of acute lymphoblastic leukemia. Cancer Cell 27:409-25
Müschen, Markus (2015) Rationale for targeting the pre-B-cell receptor signaling pathway in acute lymphoblastic leukemia. Blood 125:3688-93
Buchner, Maike; Swaminathan, Srividya; Chen, Zhengshan et al. (2015) Mechanisms of pre-B-cell receptor checkpoint control and its oncogenic subversion in acute lymphoblastic leukemia. Immunol Rev 263:192-209

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